Abstract:We studied the influence of seed size on germination, seedling growth and seedling responses to light in Ligularia virgaurea, a clonal herb native to the Qinghai-Tibet Plateau. (1) Under unshaded conditions, large seeds had significantly (P < 0.001) higher rates of germination than did small seeds. Both large and small seeds showed significantly reduced levels of germination under shaded conditions. The magnitude of this effect was greater for small seeds than for large seed. (2) Seedlings from large seeds had significantly higher rates of biomass accumulation (g·day^-1) than did seedlings from small seeds. The total biomass of seedlings from larger seeds is larger than that from smaller ones. And seedlings from large and small seeds also differed in biomass allocation. (3) Seedlings from small seeds have higher relative growth rates (RGR; g·g^-1·day^-1) than do seedlings from large seeds under both shaded and unshaded conditions. In contrast, there was no significant difference in leaf area ratio (LAR), specific leaf area (SLA) or leaf weight ratio (LWR) between seedlings from small and large seeds. RGR, LAR, SLA and LWR were all significantly higher in seedlings grown under shaded conditions than under unshaded conditions.

Abstract:The values and variation characteristics of energy components, their relationship with net radiation and the characteristics of water balance in the forest were analyzed, based on the observation data of energy fluxes, meteorological parameters and biomass in a tropical seasonal rain forest in Xishuangbanna from January 2003 to December 2004. The results show that annual net radiation was 3516.4 MJ/(m² ·a) and 3516.6 MJ/(m²·a) in 2003 and 2004, respectively, of which 46% and 44% were used in latent heat flux, and 12% and 11% were lost as sensible heat flux. Annual mean canopy surface conductance was 10.3 mm/s and 10.0 mm/s in 2003 and 2004, respectively. Moreover, canopy surface conductance was lower in dry-hot seasons than in fog-cool and rainy seasons. Canopy surface conductance correlated significantly and positively with leaf area index, but negatively with water vapor pressure deficit. In general, canopy surface conductance was not affected directly by soil water content, but highly depended on soil moisture status when soil water content was below 0.15 m³/m³. Annual total evapotranspiration of this forest ecosystem in dry seasons was lower than that in rainy seasons, which was considered as one of the most important reasons that tropical seasonal rain forest could survive and flourish in Xishuangbanna at limit of water and heat.

Abstract:The investigation on and conservation project of plant biodiversity in the inundation and resettlement districts of the Three Gorges region were carried out with the ongoing construction of the Three Gorges Dam. Based on the first-hand specimens collected for many years and the historical records, we found that there were about 1784 higher plant species, belonging to 762 genera of 170 families, which accounted for 59.19%, 75.30% and 85.85% of the total amount of species, genera and families in the Three Gorges region, including 27 species specific in these two districts. The shrub and grass communities were mostly distributed along the low-altitude river bank zone of the Yangtze River and were largely influenced by the dynamic water level of the Three Gorges reservoir area. The measurement of Shannon-Wiener and Pielou evenness indexes on 21 Pinus massoniana (Masson pine) communities distributed in the Three Gorges reservoir area was carried out, and results showed that the biodiversity of shrub layers was the highest, followed by grass layers, the middle, and tree layers, the lowest. Among the 11 typical shrub communities in this region, the abundance of shrub layers of Form. Rhus chinensis, Form. Cotinus coggygria var. cinerea, Form. Vitex negundo var. cannabifoliaand and Form. Coriaria sinica was relatively higher, which were 16, 26, 29 and 15, respectively. These four shrubs also showed high biodiversity index (H′), which were 1.791, 3.427, 2.949 and 1.718, respectively. Among the 9 grasslands mainly distributed along the river bank zone, 4 grassland communities with higher altitude (Form. Dicranopteris dichotoma, Form. Miscanthus floridutus, Form. Imperata cylindrica var. major and Form. Arthraxon hispidus) showed higher abundance and biodiversity, which were 1.697, 1.354, 1.144 and 1.018, respectively. Considering the inundation and resettlement districts, there were 22 vegetation types threatened by submersion, including 4 woody plant communities, 9 shrub communities and 9 grassland communities. The 6 shrub communities of Form. Securinega wuxiensis, Form. Bauhinia brachycarpa, Form. Geum aleppicum, Form. Distylium chinense, Form. Buxus henryi and Form. Myrsine africana would be fully submerged and part of Adianthum reniforme var. sinensis and Buxus ichangensis would be also submerged. At present, plant species conservation and monitoring stations in the Three Gorges region have been constructed, and more than 200 plant species have already been transplanted and conserved, including 37 threatened species listed in the China Plant Red Data Book and 11 local constructive species.

Abstract:Molecular diversity of halophilic archaea from Ayakekumu salt lake was investigated by the polymerase chain reaction (PCR) amplification and culture methods. 19 water samples and 15 soil samples were taken from 19 sites within Ayakekumu salt lake in winter and spring. Under aerobic culture conditions, some halophilic microorganisms were isolated by five different media. The 16S rRNA gene sequences of 62 red strains were amplified by using PCR, determined by the DNA sequencer and analyzed through the BLASTn program subsequently. Results revealed that all sequences belonged to six genera grouped within the Halobacteriaceae. Mostly 16S rRNA gene sequences related to the genera Halorubrum (47%) and Natrinema (24%) were detected. Subsequent analysis by using Shannon index indicated that cultured halophilic archaeal diversities are not significantly different between winter and spring samplings in Ayakekumu salt lake. Similarity values of haloarchaeal 16S rRNA gene sequences to known sequences were less than 97%, suggesting the presence of two novel taxa. In addition, taxonomic characteristics of Natrinema altunense and Halobiforma lacisalsi isolated from Ayakekumu salt lake had been described previously. The discovery of the novel species provides new opportunity to further examine the diversity of these halophilic microorganisms in Ayakekumu salt lake.

Abstract:The keys of studying urban sustainable development are material metabolism flux and efficiency. Metabolism flux of urban materials can only reflect the metabolism velocity, while its eco-efficiency can determine the metabolism capacity to support socio-economic development. The general model and the measure model of the eco-efficiency were set up, based on the source recycle (decreasing the consumption of crude resources) and the terminal recycle (decreasing the discharge of pollutants) of production and life. These models were employed to study material metabolism flux and efficiency in Shenzhen, China. Results showed that water, energy and waste metabolism fluxes have increased since 1998 with constant socio-economic development, and their eco-efficiencies have also increased rapidly. When GDP rose by 2.7 times, the metabolism fluxes of urban water and electricity rose by 1.5 and 3.0 times, respectively. When the added value of industry rose by 3.7 times, the metabolism fluxes of industrial water, electricity, energy and waste rose by 1.9, 3.5, 2.7 and 2.0 times, respectively. When population rose by 1.5 times, the metabolism fluxes of residential water and electricity rose by 1.8 and 1.7 times, respectively. During the period, the resource efficiency, environmental efficiency and eco-efficiency rose by 1.8, 3.7 and 2.3 times, respectively. Whereas the efficiency of material metabolism has been improved in Shenzhen, the scarcity of material resources has become more and more serious. Therefore, it is necessary to further improve the efficiency of material metabolism. The keys of improving the eco-efficiency of urban material metabolism are the increasing of resource and environmental efficiencies, and the establishing of the recycling chain of re-utilization of waste resources.

Abstract:Contributions of soil micro-fauna (protozoa and nematodes) to rhizosphere ecological functions and possible modes of action were reviewed. Micro-fauna in rhizosphere play an important role in release of plant available nutrients, accumulation and stabilization of soil organic carbon, hormonal effects on roots, microbial diversity and functional stability, multi-trophic interactions above ground, and bioremediation of contaminated soils. Selective grazing, active dispersal and excretion by the micro-fauna not only benefit the rhizosphere ecological functions, but also have an impact on the whole soil and above-ground community. It appears that mechanically understanding rhizosphere ecological functions would remain incomplete without considering the interactions of micro-fauna with microorganisms and roots.

Abstract:Teleconnection between the early immigration of brown planthopper (BPH) and El Nino/Southern Oscillation (ENSO) indices from January of two years previously to the current June was investigated to make long-term forecast. The teleconnection results were as follows: ENSO indices which were significantly correlated with the early immigration of BPH were primarily sea surface temperature anomalies (SSTA) in N3, N4 and N3.4 regions, accounting for 71.8% of the total. Significant ENSO indices from two years and one year before the immigration events had a proportion of about 84%, while those in the current year only accounted for 16.7%. There was significantly negative correlation between the early immigration of BPH and SSTA in each Nino region from two years before to the previous spring, whereas there was significantly positive correlation between these two factors during the period from the previous winter to the current spring. The significant correlation between the early immigration of BPH and SSTA in each Nino region in the last summer and autumn did not show any obvious tendencies. The relationship between the southern oscillation index (SOI) and the early immigration of BPH was opposite to that between the immigration and SSTA in each Nino region. The above mentioned significant ENSO indices were used as key factors to build forecasting models for the early immigration of BPH by step-wise multiple linear regression analysis. Finally, 12 integrated forecasting models were obtained, which could make predictions 3–27 months ahead and had a predictive accuracy of 88.9%.

Abstract:Apostichopus japonicus is a kind of temperate sea cucumbers, known to aestivate when water temperature rises above 20°C to 24.5°C. In this study, we measured the effects of aestivation on the energy utilization (i.e., energy allocation in growth, feces discharge, respiration and excretion) of A. japonicus with two different body weights (134.0 g ± 3.5 g and 73.6 g ± 2.2 g) at water temperature from 10°C to 30°C with an interval of 5°C. Noticeable variation in the energy utilization of sea cucumbers was observed in this study. During the non-aestivation period, energy deposit in growth was lower and the energy loss in feces accounted for the majority of the feeding energy. Under the threshold temperature, the feeding energy reduced and the proportion of energy deposit in growth became negative. During aestivation, sea cucumbers discontinued feeding energy, resulting in weight loss. Our study suggested that the ecological implication of aestivation in this species could lead to a model of energy saving during the long-term hot period.

Abstract:The vertical accumulation of heavy metals and the pollution status in Wanggang China tidal flat were studied. The base value of 210Pb in Wanggang area was obtained as 1.16 dpm/g. The fractal theories were led into the quantitative study of pollution issues. The modern average deposition rate in Wanggang area is 4.13 cm/a, according to the ²¹⁰Pb analysis. Through the correlation analysis between heavy metals, it is educed that the Fe, Cu, Pb, Zn and Li have better correlation coefficients in the area. After normalization, the nomalization values of Cu and Zn remain stable in the past 15 years. Cu shifts its value from 0.4 to 2, and Zn from 1.5 to 2.6. The information dimension of the normalized heavy metals is high in the area with loose systematic structure and lower organizational degree. The fractal dimension value of Zn moves from 3 to 6.5 with an average of 4.68; contemporary Cu from 5 to 6.5 with an average of 5.8085. The study shows that the heavy metal distribution in the area is mainly controlled by local geochemistry character with limited contribution from human activities. Meanwhile, the fractal dimension value of Pb normalization is lower, and its fractal dimension varies from 2 to 5.5 with an average of 3.608. Higher levels of self-organization of Pb mean a certain degree of lead contamination.

Abstract:Two Elsholtzia haichowensis Sun populations, one from Cu mine sites (Tonglushan) and the other from uncontaminated sites (Hong'an) in Hubei Province, China, were investigated in hydroponic experiments for the differences of Cu accumulation and ecophysiological responses to Cu under Cu treatment. The results showed that a significant increase in malondialdehyde (MDA) concentration in roots was observed in Hong'an population, whereas no significant change was observed in Tonglushan population. The root pressure exudates (ml) decreased significantly in Hong'an population, but increased significantly in Tonglushan population at high Cu concentration (≥ 20 μmol/L) treatment. Cu concentration in roots of Hong'an population was significantly higher than that in Tonglushan population. For example, Cu concentration in Hong'an population was 3 times and 4 times higher than that in Tonglushan population after 20 μmol/L Cu treatment for one day and three days, respectively. Cu concentration in Hong'an population was 20 times and 5 times higher than that in Tonglushan population after 80 μmol/L Cu treatment for one day and three days, respectively. The intracellular and secreted acid phosphatase (APase) activities in roots were significantly higher in Tonglushan population than in Hong'an one. The intracellular APase activities in Tonglushan population were 3 times higher than those in Hong'an population after 80 μmol/L Cu treatment for three days and five days, respectively. The root-secreted APase activities in Tonglushan population were 1.6 times and 1.8 times higher than those in Hong'an population after 80 μmol/L Cu treatment for three days and five days, respectively. In conclusion, excessive Cu caused serious oxidative damage in roots of Hong'an population, but not in Tonglushan population. The high intracellular and root-excreted APase activities in Tonglushan population might play an important role in reducing Cu accumulation in roots and maintaining normal phosphorus physiological metabolism in root cells under Cu stress.