The effects of nitrogen (N) deposition on Pogonatum cirratum (Sw.) Bird. subsp. fuscatum (Mitt.) Hyvoenen, a species of moss widely distributed in South China, were investigated. Plots subjected to a gradient of N treatments were established in 3 habitats with different illumination and humidity conditions. N was sprayed onto plots with NH₄NO₃ solutions, at doses equivalent to 20, 40 and 60 kg N ha^-1, in 4 applications during May 2006 and January 2007. The results suggest that high N deposition affects both carbon (C) and N metabolism of P. cirratum subsp. fuscatum, as well as the interaction between them. On the other hand, the sensitivity of the moss varied according to the humidity and light conditions. Moss in a habitat with high humidity and moderate light was more tolerant to an N deposition increase than moss in a habitat with low humidity and low light, as the latter was extremely sensitive to an increase in N deposition.