The effect of traffic and railway construction on the activities of wild animals during the daytime along the Qinghai-Tibetan highway between Budongquan (3517′ N; 9316′ E) and Wudaliang (3513′ N; 9304′ E) was studied in August 2003 and August 2004. Furthermore, passageways cross the Qinghai-Tibetan railway were monitored to determine the relationship between the usage frequency of the passageways and the distance to the Qinghai-Tibetan highway and the dimension of the passageways. The results showed that the traffic during the daytime had some effects on the Tibetan antelope (Pantholops hodgsoni), Tibetan gazelle (Procapra picticaudata) and Kiang (Equus kiang) when they were crossing the road, and especially it is significant on the Tibetan antelope. At the same time, they could adapt themselves to the changes in the surroundings by learning and by adjusting their behavior. Most of their activities took place in the morning in order to avoid the effects of traffic, and they could also find and use the passageways cross the Qinghai-Tibetan railway. The dimensions of the passageways, the distance to the Qinghai-Tibetan highway, the surrounding habitat, and human activities could influence the efficiency of the passageways. Most passageways cross the Qinghai-Tibetan railway could not be effectively used by the wildlife because of the short length and low height or because of human activities in the contiguous areas of the passageways. However, the wildlife could adapt themselves to the changes in the surroundings caused by the construction of the Qinghai-Tibetan railway by learning and by adjusting their behavior.