重庆铜锣山不同类型木本植物叶片光合能力参数与叶性状的种间关系
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重庆市科技局自然科学基金面上项目(CSTB2022NSCQ-MSX1121,CSTB2022NSCQ-MSX0280)


A study on the relationship between photosynthetic capacity parameters and leaf traits of different types of woody plants in Tongluo Mountain,Chongqing
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Natural Science Foundation of?Chongqing :(No:CSTB2022NSCQ-MSX1121,CSTB2022NSCQ-MSX0280)

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    摘要:

    在光合作用模型中,植物叶片光合能力通常由最大碳同化速率(Amax)、表观量子效率(AQY)、最大羧化速率(Vcmax)和最大电子传递速率(Jmax)表征。目前对于种间尺度,叶性状对不同类型植物光合能力参数影响机制的研究仍不清楚。采用Li-6800便携式光合仪于生长季旺季,测量了重庆铜锣山26种木本植物的光响应曲线和二氧化碳响应曲线,进一步计算了4种光合能力参数(Amax、AQY、VcmaxJmax),并同步测定了单位面积叶氮含量(Narea)、单位面积叶碳含量(Carea)、叶碳氮比(C ∶ N)、叶绿素含量(Chl)、叶片相对含水量(LRWC)、比叶重(LMA)、叶厚度(Lt)和叶干物质含量(LDMC)8种叶性状,探究铜锣山木本植物光合能力参数与叶性状的种间关系。结果表明:铜锣山26种木本植物Amax、AQY、VcmaxJmax的变异范围分别为3.35-18.85 μmol m-2 s-1,0.009-0.066 mol/mol,10.05-81.89 μmol m-2 s-1和33.45-140.92 μmol m-2 s-1。光合能力参数平均种间变异系数为47.7%,其种间变异程度高于叶性状(26.9%)。叶片光合能力在乔木和灌木植物间没有显著差异,落叶植物的光合能力显著高于常绿植物。光合能力参数与Narea和LRWC呈显著正相关,与C ∶ N呈显著负相关。Narea是估算种间植物光合能力变异的最佳叶性状,但在常绿植物中,Narea对四种光合能力参数的预测性要低于LRWC。Narea主要通过直接影响Vcmax间接影响AQY、VcmaxJmax,LRWC通过直接影响VcmaxJmax间接影响AQY和Amax。总体而言,基于Narea、LRWC和C ∶ N构建的多元线性回归模型决定系数高于任何单一叶性状预测模型,在所有植物类型条件下,其决定系数均在0.7以上,表明其具有较好的预测性。研究结果为改进亚热带地区常绿落叶阔叶林植物的光合能力估算模型提供理论依据。

    Abstract:

    In the photosynthesis model,the photosynthetic capacity of plants is typically characterized by the maximum carbon assimilation rate (Amax),apparent quantum yield (AQY),maximum carboxylation rate (Vcmax),and maximum electron transfer rate (Jmax). The mechanisms by which leaf traits influence photosynthetic capacity parameters of different types of plants at the interspecific scale remain unclear. In this study,we used a Li-6800 portable photosynthesis analyzer to measure the light response curves and carbon dioxide response curves of 26 woody plants in Tongluo Mountain during the peak growing season. Four photosynthetic capacity parameters (i.e.,Amax,AQY,Vcmax, and Jmax) were further calculated,and eight leaf traits,including leaf nitrogen content per area (Narea),leaf carbon content per area (Carea),ratio of leaf carbon to nitrogen content (C ∶ N),chlorophyll content (Chl),leaf relative water content (LRWC),leaf mass per area (LMA),leaf thickness (Lt),and leaf dry matter content (LDMC) were simultaneously measured. These measurements aimed to explore the interspecific relationships between photosynthetic capacity parameters and leaf traits of woody plants in Tongluo Mountain. The results showed that the variation ranges of Amax,AQY,Vcmax,and Jmax of 26 woody plants were 3.35-18.85 μmol m-2 s-1,0.009-0.066 mol/mol,10.05-81.89 μmol m-2 s-1,and 33.45-140.92 μmol m-2 s-1,respectively. The average inter-species variation coefficient of photosynthetic capacity parameters was 47.7%,and the degree of interspecific variation was higher than the average inter-species variation coefficient of 26.9% for leaf traits. There was no significant difference in leaf photosynthetic capacity between trees and shrubs,while deciduous plants had significantly higher photosynthetic capacity than evergreen plants. The photosynthetic capacity parameters were significantly positively correlated with Narea and LRWC,and significantly negatively correlated with C ∶ N. Narea was the best leaf trait for estimating plant photosynthetic capacity,but in evergreen plants,Narea's predictive ability for the four photosynthetic capacity parameters was lower than LRWC. Narea mainly indirectly affected AQY,Vcmax,and Jmax by directly affecting Vcmax,while LRWC indirectly affected AQY and Amax by directly affecting Vcmax and Jmax. Overall,the multiple linear regression model based on Narea,LRWC,and C ∶ N showed a higher coefficient of determination than any single leaf trait prediction model. Under all plant type conditions,its coefficient of determination was above 0.7,indicating its good predictive ability. The results of this study will provide a theoretical basis for improving the estimation model of photosynthetic capacity of evergreen deciduous broad-leaved forest plants in subtropical regions.

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徐铭泽,马磊,司洪涛,姚璐,刘江,朱冬雪,王琛,毛铮,朱丹,王科,严有龙,李成.重庆铜锣山不同类型木本植物叶片光合能力参数与叶性状的种间关系.生态学报,2025,45(6):2753~2767

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