Urban ecological resilience can provide a new way of thinking to cope with the internal pressure of the long-term development of the city accumulation and the outside world impact of the uncertain risk, which is of great significance to urban ecological security development. Based on urban ecology, from the ecological resilience against strength, resilience, and the characteristics of flexibility, a potential-elastic-stability ecological resilience evaluation model was constructed to analyze the spatial and temporal variation characteristics of ecological resilience in Wenzhou city from 1990 to 2020. Based on the statistical data, geographic spatial and temporal weighted regression (GTWR) model was used to analyze the spatial and temporal heterogeneity of the influencing factors of ecological resilience, and to provide suggestions for building good urban ecological resilience in Wenzhou. The results showed that: (1) From 1990 to 2020, the urban ecological resilience index of Wenzhou increased firstly and then decreased, and the three indexes of potential, elasticity and stability showed the same trend. The decrease in ecological resilience was temporarily alleviated by improvement of stability in the late study period. (2) The ecological resilience of developed coastal areas in the east of Wenzhou was poor, while that of mountainous areas in the west and north of Wenzhou was better. The low level of ecological resilience showed a decreasing trend of central resilience and expansion to the periphery. (3) There were significant spatial and temporal differences among the influencing factors of urban ecological resilience in Wenzhou, and there were also spatial differences in the direction and intensity of fluctuations. The differences were mainly concentrated in the urban fringe counties rather than the main urban areas. The study is expected to provide reference for Wenzhou city and coastal cities of the same grade to improve urban ecological resilience and promote regional sustainable development decision-making.