The continuous increase in the human footprint has caused a significantly spatial conflict among human-land systems in plateau lake basins. Taking the Dianchi Lake Basin as an example, considering the risk of spatial conflict among the production-life-ecological space in the basin, and based on changes in land-use landscape patterns and eco-environmental quality, this study introduced a four-quadrant model and performed a hotspot analysis for risk-quality-integrated eco-environmental zoning. The results showed that: (1) The three-life spatial conflict risk types in the Dianchi Lake Basin were generally strong, the conflict curve index showed an inverted "U" shape, and the proportion of strong conflict types has decreased. The three-life space type has changed significantly, and the area of living spaces has increased by 312.23 km², mainly due to rapid urbanization within the plateau lake basin, which has transformed numerous production and ecological spaces into living spaces. (2) The overall eco-environmental quality in the Dianchi Lake Basin showed a downward trend. Spatially, with Dianchi Lake as the center, it showed a high-low-high distribution. The higher-quality and high-quality areas decreased by 136.66 km²; the lower-quality and low-quality areas increased from 699.65 km² to 930.51 km², accounting for approximately one third of the total area of the basin. (3) The four area categories used for the comprehensive risk-quality eco-environmental zoning were high-quality, good, general and bad areas. The spatial distribution continuity of the four category areas was relatively good, and continuous expansion of bad areas was the most typical. Identifying comprehensive risk-quality eco-environmental zones in combination with the three-life spatial conflict risk can further enrich the theoretical framework and methods used in eco-environmental research, serve as a reference for future studies, and provide practical guidance for the coordinated development of human-land systems in the river basin.