Dissolved organic carbon (DOC) from plant leaves and fine roots is one of the most active compounds in forest ecosystem. In this study, we chose 13 tree seedlings in mid-subtropical evergreen broadleaves forest and measured leaves and fine roots extraction by water to study DOC production and composition. We measured carbon and nitrogen concentration of leaves and fine roots (0-1 mm and 1-2 mm), DOC concentration and composition by using Fourier transform infrared spectroscopy (FTIR) in this study. Our results show that (1) Cinnamomum chekiangense has the highest carbon concentration in leaves and fine roots (0-1 mm and 1-2 mm), Podocarpus nagi has the lowest carbon concentration in leaves and 0-1 mm fine roots, and Tsoongiodendron odorum has the lowest carbon concentration in 1-2 mm fine roots. Ormosia henryi has the highest nitrogen concentration in leaves, while Podocarpus macrophyllus has the lowest nitrogen concentration in leaves. Ormosia xylocarpa has the highest nitrogen concentration in 0-1 mm fine roots, and Tsoongiodendron odorum has the highest nitrogen concentration in 1-2 mm fine roots, while LIlex chinensis has the lowest nitrogen concentration in fine roots (0-1 mm and 1-2 mm). (2) Lauraceae species such as Machilus pauhoi, Phoebe bournei and Cinnamomum chekiangense have the highest DOC concentration from leaves leaching, while Podocarpus macrophyllus and Podocarpus nagi have the lowest DOC concentration from leaves leaching. Ormosia henryi and Ormosia xylocarpa have the highest DOC concentration from fine roots (0-1 mm and 1-2 mm) leaching, while Machilus pauhoi, Phoebe bournei and Cinnamomum chekiangense have the lowest DOC concentration from fine root (0-1 and 1-2 mm) leaching. (3) The infrared spectra showed that all 13 species in leaves and fine roots (0-1 mm and 1-2 mm) have similar infrared spectra by 6 absorption peaks and 1 transmission peak. The DOC produced by the leaching of Cinnamomum chekiangense leaves has the highest absorption rate in each band, and the LIlex chinensis leaves have the lowest one. The DOC produced by the leaching of Taxus chinensis fine roots (0-1 mm and 1-2 mm) has the highest absorption rate in each band. The DOC produced by the leaching of 0-1 mm fine roots of Ormosia xylocarpa has the lowest absorption rate in each band. The DOC produced by the leaching of 1-2 mm fine roots of Castanopsis fargesii has the lowest absorption rate in each band. Our study indicated that tree species and organ types played the important roles in regulating the quantitative and structural characteristics of DOC, and these differences should be fully taken into account in relevant studies, especially in subtropical evergreen broad-leaved forests with high plant biodiversity. It has been showed climate change would result transfer of tree species, rainfall intensity and frequency, strongly affecting the amount and composition of DOC, soil microbial activities and nutrient cycling in subtropical forests. Therefore, this study will improve our understanding on carbon cycle of subtropical evergreen natural forests in the future climate changes.