The Qinghai-Tibetan Plateau sequestrates large amounts of soil carbon and plays an important role in regional ecological security barrier and global climate change. The Sanjiangyuan National Park lies on the hinterland of the Qinghai-Tibetan Plateau and has been officially established in October 2021. Accurate assessment of the density and stock of soil carbon and nitrogen is a prerequisite for scientific recognition of its ecological function and zoning management. However, such knowledge based on field surveys is currently lacking. Based on 54 field soil samples' data, combined with ecological variables such as elevation, slope, aspect, mean annual air temperature, mean annual precipitation, and mean annual normalized difference vegetation index (NDVI) from 2000 to 2018, the boosted regression trees models, one of up-to-date machine learning logarithms, were employed to explore the spatial pattern and classifications of the topsoil (0-30 cm) organic carbon (SOC) and total nitrogen (TN) density in the Sanjiangyuan National Park. The results showed that the density of SOC and TN were (5.41±3.12) kg/m² (Mean±SD, the same below) and (0.57±0.27) kg/m², respectively, and they spatially correlated very well. The spatial variations in the density of SOC and TN were both predominated by precipitation and NDVI, having accumulatively relative contributions of more than 70%. The density of SOC and TN spatially were related to precipitation and NDVI with saturated S-shaped responses. The density of SOC and TN in the Lantsang Park and the Huanghe Park were (9.39±0.89) kg/m² and (0.92±0.09) kg/m², (8.26±2.33) kg/m² and (0.80±0.20) kg/m², respectively, which were approximately twice than those of the Yangtze Park. The classifications on the density of SOC and TN have been conducted and the rank order in the Lantsang Park decreased gradually from the centers to the surrounding areas, while they decreased from the north to the south and from the southeast to the northwest in the Huanghe Park and the Yangtze Park, respectively. The stocks of SOC and TN in the Sanjiangyuan National Park were 0.60 Pg and 0.06 Pg, respectively, of which in the Lantsang Park, the Huanghe Park, and the Yangtze Park were approximately 20%, 20%, and 60%, respectively. The stocks of SOC and TN were mainly concentrated in alpine meadows and alpine steppes, which accounted for about 90% of the total reservoir. The alpine meadows and alpine steppes are thus key carriers of carbon sequestration capacity and should be taken into high priority considerations in the Sanjiangyuan National Park. These results can provide important references for the functional evaluation and zoning management of the Sanjiangyuan National Park.