The purpose of this study is to understand the distribution and seasonal variation of different types of denitrifying bacteria in the rhizosphere and non-rhizosphere of the saline plant Salicornia europaea in the Ebinur Lake Wetland, so as to provide data support for the process of soil nitrogen cycling driven by microorganisms in the process of vegetation restoration in the Ebinur Lake Wetland, a representative of desert salinization ecosystem in temperate arid area. In this study, the rhizosphere and non-rhizosphere soil samples of Salicornia europaea were collected from the Ebinur Lake Wetland in summer, autumn and spring. The diversity and community structure characteristics of nirS-type and nirK-type denitrifying bacteria were compared and analyzed by high-throughput sequencing. RDA(redundancy analysis) was used to explore the effects of soil physical and chemical factors on the diversity and community structure of denitrifying bacteria. Among the soil samples of Salicornia europaea in the Ebinur Lake Wetland, the highest genotype diversity of denitrifying bacteria in nirS and nirK was the autumn rhizosphere, and the diversity of denitrifying bacteria in each soil sample showed rhizosphere > non-rhizosphere. The nirS-type denitrifiers in rhizosphere and non-rhizosphere soil samples of Salicornia europaea belonged to Proteobacteria, Firmicutes and Actinobacteria, while the classification of nirK-type denitrifying bacteria only includes Proteobacteria, Firmicutes. Proteobacteria accounted for a higher proportion in each soil sample. Halomonas and Pseudomonas of gamma-Proteobacteria were the dominant genera of nirS-type denitrifiers shared by all soil samples, but the relative abundance in each soil sample was different. Rhizobium of alpha-Proteobacteria was a kind of nirK-type denitrifiers widely existing in the soil samples of Salicornia europaea. There were certain differences in the community structure of denitrifying bacteria among the soil samples of Salicornia europaea in the Ebinur Lake Wetland. The results of RDA showed that water content, organic matter, total nitrogen, and ammonium nitrogen had a great influence on the diversity of nirS-type denitrifying bacteria in soil samples, while water content, organic matter, total nitrogen and alkali hydrolyzed nitrogen were the main factors affecting the diversity of nirK-type denitrifying bacteria. Soil electrical conductivity, total phosphorus, total potassium, total nitrogen and alkali-hydrolyzable nitrogen synergistically affected the community structure of nirS-type denitrifying bacteria, while organic matter, available potassium, available phosphorus, pH and nitrate nitrogen were the main influencing factors of the community structure of nirK-type denitrifying bacteria.With the change of seasons, the diversity and community structure of nirS-type and nirK-type denitrifying bacteria in rhizosphere and non-rhizosphere of Salicornia europaea were changed. The denitrifying bacteria of the two genotypes, with different main genus, jointly promoted the denitrification of wetland. For the protection of wetland ecosystems, long-term and extensive soil state assessment and dynamic monitoring of soil denitrifying microbial flora are required.