In order to well understand the response of soil C and N variation to alterations in forest litter the detritus input and removal treatment (DIRT) was used to reveal the response of soil carbon and nitrogen to alterations in forest litter in Pinus yunnanensis forest in central Yunnan Plateau. Six treatments of litter input and removal were established, which were normal litter inputs (CO), no litter (NL), double litter (DL), no roots (NR), no inputs (NI), and remove the organic layer and layer A (O/A-Less) to study the response of soil carbon and nitrogen variation to alterations in forest litter Pinus yunnanensis forest. The results showed that (1) the soil total carbon storage decreased in the order:S_C(NL)=170.92 t/hm² > S_C(CO)=168.10 t/hm² > S_C(NR)=153.26 t/hm² > S_C(NI)=147.20 t/hm² > S_C(O/A-Less)=143.54 t/hm² > S_C(DL)=134.49 t/hm². The soil total nitrogen storage decreased in the order:S_N(CO)=11.83 t/hm² > S_N(NL)=9.70 t/hm² > S_N(DL)=8.70 t/hm² > S_N(NR)=8.35 t/hm² > S_N(O/A-Less)=8.21 t/hm² > S_N(NI)=8.09 t/hm². Soil total carbon and nitrogen storage decreased significantly in all treatments compared to CO, although soil total carbon storage increased slightly in NL. (2) The aboveground litter input had significant effects on soil total carbon and nitrogen in the surface layer 0-20 cm of soil, and the underground litter input had significant effects on soil total carbon and nitrogen in the deep layer 20-40 cm of soil. (3) There is a coupling relationship between soil C and N in forest systems. There was a significant positive correlation between total carbon and nitrogen of soil. Bulk density had significant effect on carbon and carbon of soil.