Land use/cover change has a direct impact on regional climate by changing the surface reflectance. It is of great significance to explore the impact of land use/cover change on climatic factors at different spatial scales. In this paper, the land use information of Songnen Plain is extracted based on human-machine interaction. And, the Matlab program is used to obtain the changes in land use intensity at different spatial scales in the research area from 1985 to 2015. On this basis, the temporal and spatial characteristics of land use intensity and its influence on climate factors in Songnen Plain were studied. ① The results show that the mean values of land use intensity at different spatial scales are 3.92, 3.92, 3.93, 3.93, and 4.34, presenting a gradually increasing trend. ② From 1980 to 2018, the average annual precipitation in Songnen Plain showed a downward trend with a decline of -9.89 mm per decade, and the annual average temperature displayed an upward trend with an increase of 0.256 ℃ per decade. ③ There is a negative correlation between land use intensity and precipitation in Songnen Plain, which means that it can restrain the increase of precipitation. There is a significant positive correlation between land use intensity and temperature in Songnen Plain, which means that it can promote the increase of temperature. In addition, with the increase of spatial scale, the inhibition or promotion of the effects are first enhanced and then weakened. ④ Partial correlation and multiple correlation analysis demonstrate that the land use intensity of Songnen Plain is significantly correlated with precipitation and temperature. The effect of land use intensity on regional climate is more obvious on the 10 km grid spatial scale.