The ecological security pattern is the basic guarantee and important way to achieve regional ecological security, which can effectively alleviate the contradiction between ecological protection and economic development. Taking Jiangsu Province as the research area, two levels of ecological sources were determined through the importance of ecological services, ecological environment sensitivity and protection level. The ecologically advantageous land-use types containing the cultivated land, forest land and water body were selected for boundary analysis respectively for correcting the resistance surface based on land-use types. The corrected resistance surface was combined with the resistance surface constructed by the spatial distribution of the population to form the comprehensive resistance surface. The ecological corridors were identified based on the minimum resistance model. Then, the provincial ecological security pattern was constructed and optimized. The results show that the primary source area of the study area is 2908.28 km², accounting for 2.00% of the total area, and the secondary source area is 7944.92 km², accounting for 9.60% of the total area. The total length of the ecological corridor is 2284.89 km. The areas where the corridors are interconnected, are the integration of the ecological elements. The overall distribution of the ecological security pattern is "four belts and four districts". This paper uses the boundary analysis of the land-use types to construct the resistance surface, which can provide new ideas for the ecological safety pattern construction method. The research results can provide a scientific basis for the regional ecological protection of Jiangsu Province.