Tree-ring widths and allometric functions were used to reconstruct above-ground biomass (AGB) and net primary productivity (ANPP) of six dominant tree species (Tianlongshan Mt.: Platycarya strobilacea, Machilus cavaleriei; Maolan: Platycarya strobilacea, Pinus massoniana; Leigongshan Mt.: Pinus armandii, Pterostyrax psilophyllus) in three typical forests of Guizhou Province (karst evergreen and deciduous broadleaved mixed forests in two sites and another site of typical evergreen broadleaved forest). Similarity and dissimilarity of AGB and ANPP of different tree species, as well as the effects of climate change in the past five decades on ANPP were compared between karst and non-karst regions. The results showed that coniferous trees presented wider mean tree-ring widths than broadleaved trees. The mean ring-widths of coniferous trees and broadleaved trees in karst regions were narrower than those in non-karst region. The trees in karst regions indicated higher AGB and larger variation ranges than those in non-karst region. The ANPP of broadleaved trees and coniferous trees in karst regions were 2.4 ±1.2 kg a^-1 individual^-1 and 4.6 ±4.1 kg a^-1 individual^-1, respectively in the past five decades, which were significantly lower than those in non-karst region (5.6 ±4.8 kg a^-1 individual^-1 and 12.4±7.7 kg a^-1 individual^-1, respectively). Tree ANPP increase in karst regions was more related to mean annual temperature than growing season precipitation, and tree ANPP increase in non-karst region showed significant relationships with both mean annual temperature and growing season precipitation. ANPP of coniferous trees was more sensitive to climate change than that of broadleaved trees in both karst and non-karst regions.