With the continuous expansion and encryption of expressway networks, the suitable habitat for wild animals is being increasingly blocked. Construction of animal passage is an effective way to alleviate the negative impacts of expressway network on wild animals. The location of animal passage is the most important factor to contribute the effectiveness of the animal passage. Taking the Quanzhou-Sanming Expressway in Fujian Province as a case, and the Neofelis nebulosa as the target animal species, an assessment indicator system of the animal habitat was constructed, then the method of expert consultation was employed to decide the relative weight of each factor. As a result, the habitat suitability of the Neofelis nebulosa was evaluated by GIS program. On the basis of the distribution of the habitat suitability, the potential moving routes of Neofelis nebulosa were simulated by the hydrological analysis module of GIS platform. To this end, the direction of water flow was used to simulate the movement route of animals. Therefore, the three ideal locations of the animal passage for Neofelis nebulosi were determined. The results show that our method can effectively simulate and determine the trajectory of the animal movement. Therefore, this study can shed a new light on the construction of suitable animal passages for effectively alleviating the barrier effect of the highways.