We used three types of white-rot fungi, namely, Fomes fomentarius, Phellinus baumiibaumii, and Phellinus igniarius, as the study materials. The colony diameters and mycelial dry weights in different culture media were measured to compare their growth characteristics. Further, the activities of five lignocellulose degradation enzymes of the fungi were detected using colorimetry. The results showed that all the fungi were fast growing types:colony growth of P. baumii baumii in Potato Dextrose Agar medium was faster than that of P. igniarius and F. fomentarius, but mycelial biomass accumulation of F. fomentarius in liquid Potato Dextrose medium was the highest, followed by P. baumii baumii and P. igniarius. The manganese peroxidase (MnP) and laccase (Lac) productions of F. fomentarius were higher than that of the other two species, while the lignin peroxidase (Lip) yields of P. baumii baumii and P. igniarius were higher than that of F. fomentarius. The F-test value of three ligninolytic enzymes expressions among the three fungi species was 3.75^*, 5.20^{* *} and 3.01^* for lignin peroxidase, manganese peroxidase and laccase respectively, and the value for manganese peroxidase reaching a highly significant level. The wood chip induced treatment had significant difference from the control, and F-test value for lignin peroxidase, manganese peroxidase and laccase was 3.84^*, 4.19^* and 5.28^* respectively. No clear variations among fungi species for the expressions of endoglucanase and exoglucanase were noted, while the expressions were obviously affected by the carbon source in the medium, and the F-test value was 3.99^* and 4.04^* for endoglucanase and exoglucanase respectively, both values reached a significant level. Target region amplification polymorphism (TRAP) was used to analyze the lignocellulose degradation enzyme gene polymorphisms in the three white-rot fungi by using 29 sets of selected primers. By using TRAP, we amplified 357 bands, including 255 polymorphic bands, with 71.43% of the total polymorphic loci percentage, where the polymorphic loci percentage of the lignin and cellulose degradation enzyme genes reached 73.77% and 68.97%, respectively. This result indicated that the lignocellulose degradation enzyme genes of the three white-rot fungi have high interspecific variations. Thus, we showed that TRAP molecular markers are useful for the analysis of variations among species of wood-rot fungi at the gene level.