To evaluate the effects of post-anthesis high-temperature (HT) and drought stress (DS) and their interactions on starch pasting properties in wheat grains, two winter wheat cultivars differing in gluten strength, Yumai 34 (a strong-gluten cultivar) and Yumai 50 (a weak-gluten cultivar) were investigated in pot experiments at the experimental farm of Henan Agricultural University (E113°35', N34°51'). The experiment used a split-plot design of two factors, with three temperature regimes and two soil water treatments. HT treatments were performed in a climate-controlled greenhouse at 38℃ for 2 days (treatment 2, T₂) or 4 days (treatment 3, T₃) at 5 days after anthesis (5 DAA), 15 DAA or 25 DAA, while the control was treated at 28℃ (treatment 1, T₁). For the two soil water treatments, the soil relative water content was maintained at (55±5)% (W₂) for DS treatment and at (75±5)% for the control. The results indicated that both HT and DS and their interactions significantly influenced starch pasting properties in the grains of the two wheat cultivars. However, the responses of pasting parameters to HT and DS were very different between the two wheat cultivars. In the strong-gluten wheat cultivar Yumai 34, DS applied at any grain-filling stage increased most parameters such as peak viscosity, final viscosity and hold-through, among which the final viscosity was significantly improved at the early (5 DAA) and late grain-filling stages (25 DAA), and the peak viscosity was significantly increased at the late grain-filling stage. In Yumai 50, DS applied at any grain-filling stage (early, middle or late) decreased peak viscosity, setback and breakdown. Drought stress applied at the early and middle grain-filling stages (15 DAA) clearly improved hold-through and final viscosity, but significantly decreased both parameters when applied at the late grain-filling stage. Heat stress significantly increased peak viscosity, final viscosity (except when applied at 5 DAA), set-back and breakdown (except when applied at 15 DAA) in grains of the strong-gluten wheat cultivar Yumai 34, while the parameter of hold-through in grains of Yumai 34 had much different responses to HT applied at various stages: it was significantly decreased in both HT treatments (both T₁ and T₂) at 5 DAA, and was decreased in T₂ (2 days HT) but significantly enhanced in T₃ (4 days HT) at 15 DAA. In the weak-gluten wheat cultivar Yumai 50, HT significantly decreased hold-through and final viscosity in the grains, but had little influence on peak viscosity and setback. From the treatment stage results, we concluded that HT applied at the middle grain-filling stage (15 DAA) had the greatest impact on pasting properties in both wheat cultivars. The analysis also indicated that interaction between HT and DS had a significant effect on viscosity parameters in both cultivars, which was much greater in Yumai 50 than in Yumai 34. The effect of HT and DS interaction on pasting properties in Yumai 50 was greater when they were applied at the early grain-filling stage compared with the middle or late grain-filling stages. From our results, we conclude that different wheat genotypes show different responses in grain pasting properties to various environmental stresses, implying that such stresses have a complex effect on starch quality in the grains of winter wheat.