Based on Sen's function approach, this paper stated the connotation of the well-being, and defined that the freedom choice and capability of the human is the connotation of the human well-being on ecosystem services, while the poverty is the limited for capability and development of the human. Ecosystem degradation and destruction will seriously threat to human well-being, especially the well-being of the poor. Conservation of biodiversity can contribute to the protection of ecosystem services, which as the core of the ecosystem, and then improve the well-being of human. Focused on the well-being of the poor whom strongly dependent on ecosystem services and effective implemented ecological compensation, that can achieve a win-win between ecological protection and human well-being improvement.