The chicken feeding in China accounts for 24% of the world total. Cu and Zn are widely used in fodder, leading to high concentrations of Cu and Zn in chicken manure. As a good organic fertilizer, chicken manure is widely applied to garden soil, causing high concentrations of Cu and Zn in both soils and vegetables. High concentrations of these metals in soils can affect animal and human health through the food chain. It is of vital importance to know the effect of chicken manure application on Cu and Zn accumulation in soils and in Brassica sinensis L., for human health risk assessment and safe production of vegetables. Bioconcentration and translocation of Cu and Zn by Brassica sinensis L. planted in chicken manure-applied soils were studied in pot experiments. Chicken manures with high concentrations of Cu and Zn (1137.3 and 1503.4 mg/kg, respectively)were applied to soils at six rates, i.e. 0, 11, 22, 44, 89 g/kg and 222 g/kg, namely 0, 25, 50, 100, 200 t/hm² and 500 t/hm², respectively. The results showed that total Cu and total Zn concentrations in manure spoiled soils ranged from 58.6 to 203.4 mg/kg and 78.1 to 431.6 mg/kg, respectively. EDTA-Cu was in the range of 12.7-119.8 mg/kg, and EDTA-Zn 15.6-215.1 mg/kg. There was a significant difference in the concentrations of total Cu and total Zn in the soils between control and the treatments above 50 t/hm². The concentrations of Zn and Cu in every part of Brassica sinensis L increased with addition of chicken manure. Futhermore, Zn concentration was higher than Cu in every part of Brassica sinensis L. Both Cu and Zn concentrations in the under-ground parts were greater than those in the above-ground parts for all treatments. The concentration of Zn in every part of Brassica sinensis L. was also higher than that of Cu. There was a significant difference in Cu concentrations in the above-ground parts of Brassica sinensis L. between control and treatments above 100 t/hm². However, no significant differences in Zn concentration were observed among all the treatments. There were significant differences in Cu concentrations in the under-ground parts of Brassica sinensis L. between control and all treatments except 25 t/hm², but in Zn concentrations in the under-ground parts of Brassica sinensis L. between control and all treatments except 25 t/hm² and 50 t/hm². Good correlation ocurred between the extractable Cu(EDTA-Cu)concentrations in soil and in Brassica sinensis L. tissues. In contrast, no significant correlation between the extractable Zn(EDTA-Zn)concentration in soil and the Zn concentration in Brassica sinensis L. tissues was found. The bioconcentration factor of Cu in Brassica sinensis L. increased from 11% to 15% when manure application rates changed from o to 222 g/kg, while that of Zn decreased from 47% to 19%. Translocation factors for Cu and Zn decreased 36% and 51% respectively,with the change of manure application rate. The decrease of the ratio of Zn:Cu in every part of Brassica sinensis L.indicates that the discrepancy in the uptake and translocation capacity of Cu and Zn in Brassica sinensis L reduced with increasing chicken manure application.