The urban planning method instructed by ecology theory emphasizing on the over-all construction in ecological, social and economic environments comprehensively, has become the important way to realize harmonious, highly effective and sustainable development of a city. This paper presents the approach to reach city′s spatial optimization and function organization based on the ecological priority principle by using the analysis method in Landscape Ecology, taking the Linhai New Town in Tianjin Binhai New Area as an example. It includes the following three steps: Firstly the city′s ecological landscape pattern was optimized, by assessing the present situation and diagnosing problem; then the urban eco-function zones were determined by analyzing the ecological suitability and sensitivity with GIS spatial analysis techniques; finally based on the above steps, the optimized spatial configuration was obtained, combing with the city′s function orientation and industry selection. Demonstrational analysis shows: the essence of urban planning founding on ecological priority benefits to natural reconstruction and reservation; the construction of eco-spatial network decides the cluster pattern of city function organization; the spatial layout based on the ecology analysis safeguards the healthily and sustainable development of city ecosystem.