The concentration of two kinds of secondary metabolite-muddy odorous compounds (geosmin and2-methylisoborneol)of algae and actinomycete in intensive cultivation ponds of saline water in Tianjin were detected by coupling solid phase micro-extraction with gas chromatography-mass spectrometry, the degree of earthy flavour of fish and biomass of the algae and actinomycete in the ponds were determined at the same time. The tripartite relationship (the concentration of geosmin and MIB, the biomass of the algae and actinomydete, the earthy flavour of fish in the ponds) was researched. It was found that geosmin and MIB existed in all the ponds, and MIB was the major odorous compounds with its concentration from 0.33 ng·L-1 to 5302.70 ng·L-1.The concentration of geosmin was less than MIB, it was from 0.29 ng·L-1 to 12.10 ng·L-1 .Algae of six phylum and ninety-four genera was determined in the ponds with its biomass from 007 ng·L-1 to 186.48 ng·L-1, and Cyanophta and Euglenophyta were dominant species. Four genera actinomycete with gross biomass from 0.01×104~1.50×104 ind.·L-1 were found in the ponds and Streptomyces was dominant. It wss found that MIB in the intensive cultivation ponds of saline water in Tianjin was mainly produced by Oscillatoria princeps and actinomycete. It was also found that the occurrence of the earthy flavour in the fish and in the water was not at the same time.