Panicle, leaf and air temperature above canopy of 15 rice varieties were measured to study the relationship between air temperature and the temperature of rice organs. The results showed that the temperature of panicle and leaf were lower than air temperature, the temperature difference between air and organ of rice (TD) was affected by air temperature, air humidity, plant type and age of organs of rice. Under conditions of similar air humidity, the TD was lower in the low air temperature (below 26.5℃) than in the high (above 33℃) air temperature. At the same air temperature, the TD decreased when the air humidity was high. The TD was also affected by the plant type of the varieties. Under similar climatic conditions, the TD of erect panicle varieties was higher than that of curved panicle varieties; The TD of the varieties whose panicle was higher than the flag leaf was higher than varieties whose flag leaf was higher than the panicle. After heading, there were significant differences between the TDs at different stages of rice growth, the more mature the organs, the higher the TD.