The Yellow Sea is a semi-closed shallow water sea in northwestern Pacific, the average depth is 44 meters. Continental climatic changes and several ocean currents including the Yellow Sea coastal currents, the Yellow Sea warm current, and the Yellow Sea Cold Water Mass result in very complicated oceanographic conditions, as well as very complex macrobenthic fauna in terms of community structure, species composition, and quantitative distribution in the Yellow Sea. A cross transection macrobenthic research was conducted in the Yellow Sea in August 2001, and August, September, and October 2002. The sediment samples were collected from 10 stations cross transection from Qingdao coast of China to the southwest coast off Korean Peninsula. Samples were grabed by 0.1 m² box corer, every two successful grabs were regarded as one sample representing a station in one cruise. 36 samples were obtained in this manner in the four cruises. Living organisms were collected by filtering samples through 0.5 mm sieve, and then fixed in 75% alcohol. The characteristics of macrobenthos cross the transection was studied and described with the indices of relative importance, Shannon-Wiener index, species richness index, species evenness index, Bray-Curtis similarity cluster, and MDS ordination. Comparing those of neighboring marine areas, the species richness index of the macrobenthos fauna was lower, while the species evenness index is higher and Shannon-Wiener index is similar. 182 species were identified, of which 54 species were Polychaeta, 29 were Mollusca, 66 were Crustacea, 17 were Echinodermata, and the rest 16 were other animal groups. The number of macrobenthos species in Stations 4 and 5 were distinctly lower than those in other stations. The Bray-Curtis similarity was used to describe the macrobenthic community structure, which shows that the macrobenthos communities in the 10 stations could be devided into three groups in temperature: eurythermal, microthermal, and mesothermal communities. The species composition among stations was very defferent between the data of 2001 and 2002, and no clear change was observed in the composition from August, September, and October, 2002.