All living and production activities are based on resource consumption and these activities greatly affect the environment. The activities of tourism also generate such enduring environmental affects. The eco-footprint analysis of tourism is one of the most up-to-date and effective methods used to analyze the effects of tourism. In this paper, the tourism line product of Shangri-La, Yunnan, was studied as a case product. According to the characteristics of this line product and based on wide reference of relevant eco-footprint reports, the revised calculation method of the eco-footprint for itinerary production was used in our investigation. The typical 8 day tour′ of the Shangri-la Tourism Line was analyzed and its eco-footprint was calculated by this revised method. The difference between this revised calculation and the former calculation is that the revised calculation focuses on the traveling route, while the latter method based calculations on all data within the specific area. The results showed that: (a) the composition of Tourism Itinerary products eco-footprint basically consisted of four components, which are food consumption, living accommodation, travel and solid wastes. Under the constant condition of other factors, the eco-footprint of the tourism line product is primarily caused by travel activities. On a similar kind of tour, there is a relationship between the spending of tourists and the different components of the eco-footprint, but it is not proportional. (b) According to composition, the ecological consumption of the tourism activity consisted of seven parts, and it was proved by real diagnosis research that the major four are food consumption, living accommodation, travel and solid wastes. The tourist ecological footprint is larger than the daily per capita consumption of nature resources, and it is far higher than the daily per capita consumption of local populations residing in the tourist destinations.（c）The components of the ecological footprint and the ratio that counter balances all of consumer expenditure share a non-linear relationship. The travel component ratio of the ecological footprint is higher, and the travel expenditure ratio of all the expenditures is lower. The ratio of the ecological footprint for the three components of sightseeing, entertainment, and accommodation, are below the corresponding share of all consumers spending.