During the investigations in several years, the transgenic insect-resistance hybrid poplar 741 presented a continuance resistance on target pests, lepidopter, causing significant decrease in pest populaton. There was no obvious impact on the population of pest between high and medium insect-resistance hybrid poplar 741 stands. Transgenic insect-resistance hybrid poplar 741 had negative effect on target and non-target phytophagous pests, but had positive effect on natural enemies and neutral arthropod. These results demonstrated that different control strategy and measure should be taken in integrated pest management of transgenic insect-resistance hybrid poplar 741, compared to those of the parental hybrid poplar 741, and the high insect-resistance clones had better be used as plantations for the ecological regulation.