The paper constitutes a study on the effect of algae on population dynamic of rotifers (Brachionus calyciflorus) at the temperature of (28±1)℃, under the intensity of illumination 200～250lx, illumination period L∶D=12∶12, and the same biomass of algae. Rotifers are cultured by feeding on Chlorella pyrenodeosa(C.p), Anabaena flos-aquae(A.f), Chlamyclomoras sajao(C.s) and mixtures(wet weight) of them at proportion 3∶1, 1∶1 and 1∶3 using population accumulative culturing method. The results show the difference in population density of rotifers feeding on C.p, A.f and C.s under different concentrations is very significant (p<0.01), significant (p<0.05) and not significant (p>0.05) respectively. Moreover, the cultivation effect of rotifers feeding on the mixture of C.p and A.f under 3 proportions is superior to (p<0.05) that of any corresponding alga. Whenever rotifers are fed on single C.s or its mixture, the cultivation effect is inferior without exception. Therefore, C.s is not suitable for developing food for rotifers. The study also indicates that C.p is the best for the growth of the rotifer among the three algae species, and the mixture of C.p and A.f is more suitable for cultivating rotifers than single C.p, which can offer reference for developing food of rotifers.