高寒矿区人工草地恢复过程土壤理化性质与细菌群落的交互影响
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青海威斯特铜业有限责任公司德尔尼铜矿生态修复工程生态监测服务项目(2023-2024年); 高等学校学科创新引智计划项目(D18013)


Interactive effects between soil physicochemical properties and bacterial community during artificial grassland restoration in alpine mining areas
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    摘要:

    独特的气候条件和地理位置导致高寒矿区生态修复工作极具挑战性、自我恢复能力有限,人工草地建植成为关键的生态修复措施。研究人工草地修复过程中的土壤理化性质和细菌群落特征,对于评估修复效果和制定管护措施至关重要。采用空间代替时间的方法,以青海省玛沁县德尔尼矿区排土场边坡不同恢复年限的人工草地为研究对象,并以天然草地作为对照,分析了土壤理化性质和细菌群落特征的时间动态变化,同时对比了人工草地与天然草地的差异。研究结果如下:恢复2年后,土壤养分含量和细菌α多样性指数显著增加。然而,随着恢复时间的延长,这些参数均呈下降趋势,包括土壤养分、土壤含水量、pH值和α多样性指数。不同恢复年限的人工草地与天然高寒草甸在土壤有机质、全氮、有效氮、pH值和土壤容重方面存在显著差异(P<0.05)。不同恢复年限下细菌群落的β多样性差异显著(P<0.05)。随着恢复年限的增加,人工草地与天然草地细菌群落结构的差异性逐渐减小。在不同恢复阶段,优势菌门无显著差异,变形菌门(Proteobacteria)和放线菌门(Actinobacteria)占细菌总操作分类单元(OTUs)的50%以上,而厚壁菌门(Firmicutes)的相对丰度则随恢复年限的增加而下降。相关性分析表明,细菌多样性对土壤因子的响应具有时间差异性:α多样性受土壤有机质、全氮、土壤含水量和pH值的显著影响,且物种丰富度主要受氮限制。此外,门水平的细菌丰度与特定的土壤理化性质存在显著相关性,这证实了细菌群落结构与土壤特征之间存在强烈的交互作用。

    Abstract:

    The unique climatic conditions and geographical location resulted in challenging ecological restoration and limited self-recovery capacity in alpine mining areas. Artificial grassland establishment emerged as a critical ecological restoration measure. Investigating soil physicochemical properties and bacterial community characteristics during artificial grassland restoration was essential for evaluating restoration efficacy and developing management strategies. Using a space-for-time substitution approach, this study selected artificial grasslands with different restoration duration on dump slopes in the Delny mining area (Maqin County, Qinghai Province) as research subjects, with natural grassland as control. The investigation analyzed temporal changes in soil physicochemical properties and bacterial community characteristics, comparing artificial versus natural grasslands. Key findings revealed that: After 2-year restoration, soil nutrient content and bacterial α-diversity indices increased significantly. However, these parameters declined with prolonged restoration, including soil nutrients, soil water content, pH, and α-diversity indices. There were significant differences in soil organic matter, total nitrogen, available nitrogen, pH and soil bulk density between artificial grassland with different restoration duration and natural alpine meadow (P<0.05). The β diversity of bacterial community in different restoration duration was significantly different (P<0.05). With increasing restoration duration, the structural dissimilarity between artificial and natural grassland bacterial communities progressively decreased. No significant difference occurred in dominant bacterial phyla across recovery duration. Proteobacteria and Actinobacteria comprised over 50% of total bacterial OTUs, while the relative abundance of Firmicutes declined with increasing recovery duration. Correlation analysis revealed temporally divergent responses of bacterial diversity to soil factors: α-diversity was significantly influenced by soil organic matter, total nitrogen, soil water content, and pH, with species richness primarily nitrogen-limited. Furthermore, phylum-level bacterial abundances showed significant correlations with specific soil physicochemical properties, confirming strong interactive effects between bacterial community structure and soil characteristics.

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王克宙,史兴萍,李希来,张明,尚卿,周怡志.高寒矿区人工草地恢复过程土壤理化性质与细菌群落的交互影响.生态学报,2026,46(6):2846~2860

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