The phenomenon of insect pheromones triggering host resistance had been well-documented across various research systems, which was important for identifying and screening plant resistance elicitors to counteract pest damage. This study examined the effects of treating Picea koraiensis with the aggregation pheromone of the Ips typographus on the colonization of the pathogenic fungus Endoconidiophora polonica. The primary goal was to identify chemical compounds that could induce resistance in P. koraiensis against this pathogen. The aggregation pheromones 2-methyl-3-butene-2-ol and S-cis-verbenol were injected into the phloem of spruce, and E. polonica was inoculated on the 1^st day and 30^th days after treatment. The lesion size of E. polonica in the phloem was measured at 60dpi, and the monoterpenes contents at the inoculation site were analyzed to determine their effect on fungal growth. Our results showed the aggregation pheromone treatment could not inhibit the lesion size of E. polonica in phloem of P. koraiensis after 1 day treatment. But 2-methyl-3-butene-2-ol treatment significantly inhibited the colonization of E. polonica in P. koraiensis after 30 days treatment, and the induced resistance of P. koraiensis to E. polonica was similar to that induced by the plant hormone methyl jasmonate. After treatment with 2-methyl-3-butene-2-ol for 30 days, the amount of total monoterpene increased significantly after interaction with E. polonica. Laboratory bioassay showed that all monoterpenes could inhibit the growth of E. polonica. The aggregation pheromones 2-methyl-3-butene-2-ol could induce the P. koraiensis resistance against pathogenic E. polonica. The up-regulation of the defensive substance monoterpene after the interaction between P. koraiensis and E. polonica was the key factor to inhibit the colonization and growth of this pathogenic fungi.