The spatial dynamics of ecosystem services are characterized by an imbalance between supply and demand, which drives the flow of these services and significantly affects regional ecological security and the sustainable development of the social economy. It is critical to clarify the process and intensity of spatial flow. Taking Guangdong Province as a typical case area, where the trade-off game between rapid economic development and ecological environment protection is prominent, and comprehensively evaluates its main ecosystem service types and supply capacity based on InVEST, RUSLE, CASA and other models. The study evaluates its demand capacity from the dimensions of land use, population and economic development, and analyzes the spatial matching characteristics of its ecosystem services supply and demand. The breaking point model and field strength model are constructed to reveal the flow process, scope and flow intensity of ecosystem services. The results showed: (1) From 2000 to 2020, the regional differences in the overall ecosystem services supply and demand capacity of Guangdong Province were significant, which was consistent with the spatial development orientation of ‘one core, one area and one belt’. The ecological services supply capacity of the northern ecological barrier zone was significantly stronger. With the continuous economic development and the improvement of residents ' consumption level, the Pearl River Delta region has formed a core high-value area of ecological services demand, and has shown a pattern of decreasing radially to the periphery. (2) The ecological services in Guangdong Province are generally in a stable state of slightly oversupply. The Guangzhou-Foshan urban belt and the Shenzhen-Dongguan urban belt are obviously low-value areas of supply and demand ratios. The western and northern Guangdong regions are the main supply areas of grain production services and water yield services, the northern Guangdong region is the main supply area of carbon sequestration services, and the soil conservation services are the main supply in eastern and northern Guangdong. (3) The flow of ecosystem services in the province has formed a demand area dominated by urban agglomerations on both sides of the Pearl River Delta estuary, and the inflow of ecosystem services is the mainstay. The eastern and western Guangdong are mainly evacuated and supplied outflow. The Pearl River Delta region can be covered by the maximum flow range of most supply areas, and its ecosystem services flow intensively, with high average flow intensity and obvious siphon effects; There are also inflows in the central urban areas of western and eastern Guangdong, but the flow intensity and density are lower than those in the Pearl River Delta region. The spatial difference of ecosystem services produces the spatial relationship between supply and demand. The spatial difference of ecosystem services flow is affected by the remote coupling of nature, social economy, land use, stakeholders and multiple factors.