Nature's value constitutes a foundational framework for analyzing human-natural resource interdependencies and well-being linkages. How tourism can better generate the value of nature is an inherent demand for the high-quality development of protected areas and the realization of community human well-being in the context of the transformation of the management system. Integrating ecosystem service theory, IPBES (Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services) framework, and nature's relational values, this paper constructs a theoretical framework for the relationship between the value of nature in protected areas and human well-being under the intervention of tourism, and conducts empirical research on three community archetypes within Huangshan's national park pilot zone: Agricultural-dominant (AD), Agri-tourism-integrated (ATI), and Tourism-dominant (TD), namely, Agriculture-led community, Agri-tourism-integrated community, and Tourism-led community. (1) The characteristic analysis shows that in Agriculture-led communities, the production level of the instrumental value of nature is relatively low, and the shaping of relational value is weakened, resulting in the problem of insufficient well-being; in Agri-tourism-integrated communities, the production of the instrumental value and relational value of nature gathers at a relatively high level, and well-being is relatively balanced; in Tourism-led communities, the spatial differences in the production of the instrumental value of nature are significant, and the distribution of economic relational value varies greatly, leading to the polarization of well-being. (2) The mechanism analysis reveals that the traditional production mode of the value of nature dominated by agriculture helps maintain the basic material guarantee and social relationship foundation required for human well-being, but its production efficiency is limited and it cannot meet the needs of improving human well-being. Tourism development strategies must synergize with traditional value utilization pathways while preserving biocultural heritage, maintain the basis of the natural state and social atmosphere; enrich the production of the instrumental value of nature through different business forms, and strengthen the shaping of non-economic relational value through leisure opportunities, so as to achieve a balanced improvement in human well-being.