Biodiversity mainstreaming has garnered significant international attention and consensus as a pivotal strategy to address the escalating crisis of biodiversity loss. In response to the Kunming-Montreal Global Biodiversity Framework, which underscores the necessity of integrating biodiversity considerations into broader policy frameworks and innovating policy tools and solutions, the Chinese government updated and released the National Biodiversity Strategy and Action Plan (NBSAP) in 2024. The NBSAP prioritized biodiversity mainstreaming as the primary task, and established business biodiversity conservation as a priority action for the first time, with the aim of guiding enterprises to play an active role in jointly curbing biodiversity loss. This study addresses critical challenges hindering business biodiversity conservation in China, including regulatory framework deficiencies, inadequate disclosure mechanisms, absence of standardized technical protocols, and insufficient scientific support systems. Through a tripartite methodological approach combining systematic literature review, cross-jurisdictional comparative analysis, and in-depth case study deconstruction, the research comprehensively investigates global evolutionary trends in business biodiversity governance paradigms and policy instrument innovation. Particular emphasis is placed on three emblematic international models: the European Union's comprehensive biodiversity legislation framework, the United Kingdom's pioneering Biodiversity Net Gain (BNG) policy , and Brazil's innovative Private Natural Heritage Reserves (RPPN) system demonstrating private sector engagement. The analytical framework reveals four strategic pathways for enhancing China's corporate biodiversity governance: (1) construct an integrated corporate biodiversity conservation framework that spans the entire lifecycle;（2）establish a corporate biodiversity disclosure mechanism that combines mandatory requirements with incentive-based approaches;（3）refine the sustainable utilization mechanism of biodiversity based on the value realization of ecological products;（4）cultivate a collaborative governance mechanism involving multi-party cooperation among the government, enterprises and communities.These recommendations are designed to address the existing challenges and to foster a more comprehensive and effective approach to business biodiversity conservation in China, ultimately contributing to the global effort to halt biodiversity loss and promote sustainable development.