The cultural service value of ecosystems can bridge the connection between ecosystems and human society, bringing non-material well-being and benefits to human society, and playing a unique role in the construction of ecological civilization, which has attracted attention from the academic community. Desert tourism scenic area play an important role in the northern tourism cultural geography system, but the academic community has not paid enough attention to the evaluation objects of ecological system cultural services in desert tourism scenic area, it results in the inability to theoretically understand the characteristics, rules, drive power, and impact mechanisms of ecological system cultural services in desert tourism scenic area. At the same time, it also leads to the overall low spatiotemporal response ability of desert tourism scenic area to the current national major cultural development strategy, and the inability to effectively release cultural service functions. This study notes that desert tourism scenic area are different from general natural scenic area and historical cultural scenic area, it emergs the “dual fragility” characteristics of natural ecology and cultural ecology. Under the superimposed effect of ecological civilization construction, cultural strengthens the country, and cultural tourism integration strategies, make the desert “culturally” has become a new problem that needs to be solved in desert ecological governance. Taking the Tukai desert tourism scenic area as the research object, this study uses multi-source data from field research, online questionnaires, and self media platform comments to construct an evaluation index system for the cultural ecosystem services value of the scenic area based on tourist perception, and Using ArcGIS 10.8 as a research tool,produces a heat map of the cultural ecosystem services value of the scenic area by Natural Breaks Method; Use the average nearest neighbor to analyze the spatial agglomeration characteristics of cultural ecosystem service value, and use a geographic detector to analyze the environmental impact of cultural ecosystem service value spatial agglomeration. The research results indicate that there are three problems with the cultural ecosystem service value of scenic area: (1) spatial layout and incomplete value chain incomplete; (2) Weak spatial correlation; (3) The environmental impact mechanism is unreasonable. The research has developed a theoretical framework and method for evaluating the cultural ecosystem service value of desert tourism scenic area, which can provide reference for the development of cultural ecosystem service value of desert tourism scenic area and improve tourists’ non-material well-being, and enhance the spatiotemporal response capability of desert ecosystems to the current major cultural strategies.