Ecotourism comfort serves as a vital avenue for fostering harmonious coexistence between humans and nature, embodying the principles of harmonious coexistence and the "Two Mountains" theory. Grounded in the theory of urban comfort, this study assesses the level of ecotourism comfort in Chengdu from 2010 to 2023. Utilizing the Minimum Cumulative Resistance (MCR) model and the Gravity Model, we extract potential ecotourism comfort corridors to pinpoint the core areas. The configuration paths are explored through fuzzy-set Qualitative Comparative Analysis (fsQCA). Our findings reveal that: (1) The level of ecotourism comfort in Chengdu has seen an overall improvement, featuring a "core-periphery" spatial structure with "polarization leading and common development." Notably, Wuhou District, Qingyang District, and Longquanyi District stand out as core areas, exerting a significant influence on the periphery; (2) The distribution of potential eco-corridors in Chengdu exhibits a "dense east, sparse west" pattern, with core ecotourism comfort corridors radiating primarily from Xinjin District and Longquanyi District, showing a banded pattern and clustering in the peripheral areas; (3) The high level of ecotourism comfort in Chengdu is driven by diverse pathways, primarily categorized into five types: "comprehensive drive, resource-transportation integration, technology-transportation synergy, financial investment focus, and tourism market-technology fusion." These findings offer a theoretical foundation and scientific reference for enhancing the level of urban ecotourism comfort and promoting the sustainable development of ecotourism comprehensively.