Urban heat island and flood have become the most prevalent and widespread ecological issues that cities cannot afford to overlook. Effectively devising adaptation measures and mitigation strategies for urban heat island effects and flood is a shared challenge for urban planners, climatologists and geographers. Under the background of rapid urbanization, the relationship between urban heat island effect and flood has been inseparable. This study aimed to elucidate the spatial linkages between heat mitigation and flood regulation services, delineate various ecosystem management zones, and propose corresponding strategies, thereby offering a theoretical basis for mitigating urban heat islands and flooding in Wuhan. The InVEST(Integrated Ecosystem Services Assessment and Tradeoff) model, surface temperature inversion and SCS-CN(Soil Conservation Service Curve Number) model were used to measure the supplies and demands of heat mitigation and flood regulation services in Wuhan from 2000 to 2020. Additionally, the degree of supplies and demands were also calculated by the supply and demand ratio. The trade-off/synergy relationships during supplies or demands between heat mitigation and flood regulation services were analyzed by Pearson correlation coefficient and Moran index from 2000 to 2020 in Wuhan. Finally, Wuhan were divided into six ecosystem management zones based on the trade-off/synergy results of supplies and demands from 2000 to 2020. The results are as follows:(1) From 2000 to 2020, the areas of flood regulation and heat mitigation services in short supply expanded from the central urban area, and the expansion range of flood regulation services was larger. (2) Overall, the supplies or demands of flood regulation and heat mitigation services from 2000 to 2020 showed different degrees of synergies. Locally, it showed that the synergistic scope of supplies or demands of flood regulation and heat mitigation services in the central urban area expanded outward from 2000 to 2020, and the trade-off scope shifted outward. (3) On the basis of supply and demand matching and tradeoff/synergy effect, Wuhan City was divided into 6 ecosystem management zones, including "insignificant zone of demand", "insignificant zone of supply", "supply and demand synergy zone", "supply tradeoff demand synergy zone", "supply synergy demand tradeoff zone" and "supply and demand tradeoff zone". Based on the distinct ecosystem management zones identified in 2020, tailored ecosystem management strategies were recommended following an analysis of the supply and demand balance for heat mitigation and flood regulation services. This study has increased our understanding of the relationship between heat mitigation and flood regulation services, and provided a new perspective for the joint study of urban heat island and flood. The division of ecosystem management zones and the proposed strategies has theoretical guiding significance for mitigating urban heat island and flood.