Constructing an ecological security pattern is crucial for the ecological protection and restoration of territorial spaces. Exploring the construction of ecological networks based on various theories, identifying key nodes, and clarifying their characteristics, as well as innovating a multi-method coupled work system, holds significant importance for ecological protection and restoration of territorial spaces. This paper uses Jining City as an example, taking the natural protected areas of Jining as ecological sources. Based on the migration characteristics of terrestrial organisms, factors such as NDVI, slope, terrain ruggedness, land use type, distance from roads, and distance from water bodies were selected as resistance factors. A comprehensive resistance surface was generated using a weighted overlay, which was obtained through principal component analysis. The MCR-gravity model and circuit theory were applied to identify ecological corridors (EC), ecological protection nodes (EPN), and ecological restoration nodes (ERN). Five parameters-network closure degree, line-point ratio, network connectivity, cost ratio, and key node density-were used to evaluate the differences in ecological network structures based on the two methods. Additionally, the landscape types, spatial distribution, and node locations contained within were compared. The research results are as follows: (1) Based on the MCR-gravity model, 51 ecological corridors (EC), 121 ecological protection nodes (EPN), and 58 ecological restoration nodes (ERN) were extracted and identified. Using circuit theory, 50 ecological corridors (EC), 232 ecological protection nodes (EPN), and 208 ecological restoration nodes (ERN) were identified. (2) The ecological network identified by the MCR-gravity model exhibits a more complex and closed structure, with higher stability of ecological corridors. Circuit theory has an advantage in identifying key nodes. (3) The landscape types contained in the ecological networks identified by both models are similar, mainly consisting of farmland and forest-grassland, indicating that developing ecological agriculture is of great significance for the ecological security of grain-producing plain areas. The MCR-gravity model's ecological corridors contain more forest land. Circuit theory can better identify ecological nodes within urban development boundaries and non-controlled areas, effectively improving the success rate of biological migration. These results provide guidance for the ecological protection and restoration of territorial spaces in Jining City.