Abstract:To better understand the influences of shellfish release on the macrobenthic community in intertidal zone, we sampled biological materials in the tidal zone of Kanmen Bay. Two sampling special schemes were used, i.e., shellfish releasing area and the control area sampled in May 2022 and October 2022. The macrobenthic communities were analyzed in the two areas. In total, 89 taxa of macrobenthos were identified across all areas, among which 62 and 59 taxa were recorded in shellfish releasing area and in the control area, respectively. Mollusca were the dominant macrobenthos in the whole area, shellfish releasing area and the control area, accounting for 46.07%, 51.61% and 49.15% respectively. In spring, there were nine dominant species in shellfish releasing area, and four in the control area. Odostomia subangulata and Amphibalanus amphitrite were common dominant species. In autumn, there were four dominant species in shellfish releasing area, and five in the control area. Assiminea latericea and Macrophthalmus (Mareotis) japonicus were common dominant species. The average abundance, biomass, Shannon-Wiener diversity index, and Margalef richness index were ranked as shellfish releasing area>control area despite whether it was in spring or in fall, while the Pielou's evenness index was higher in control area than in shellfish releasing area. Results of two-way ANOVA showed that the biomass of the macrobenthos was significantly different between the shellfish releasing area and the control area, the number of species, density were significantly different between spring and autumn, and other parameters were not significantly different between regions and seasons. All the macrobenthos were divided into two groups-T1 and T2 sections in shellfish releasing area, T3 and T4 sections in control area in spring, three groups-T1 sections in shellfish releasing area, T2 sections shellfish releasing area, T3 and T4 sections in control area in autumn. Combined with pre-release and surrounding survey data, though the species, average abundance, average biomass, Shannon-Wiener index(H'), and Margalef's species richness index (D) increase atfter two years of shellfish releasing, the recovery of macrobenthos in the study area was not satisfactory.