Nest predation is an important factor driving the evolution of bird behavior, prompting birds to evolve a series of behavioral strategies. In order to explore the behavioral strategies of birds to deal with different types of nest predators, the nest invasion experiments of Elaphe anomala and chipmunk (Tamias sibiricus) were simulated from April to July 2016. Based on the artificial nest box, the anti-predation behavior of parents and chicks of varied tits (Sittiparus varius) was studied in the early (6-8 days old) and late (15-17 days old) brooding period respectively. The results showed that the anti-predation behavior of parents and nestlings of varied tits would be adjusted according to the change of breeding process and predator type. The intensity of parent bird's response to predator behavior increased with the advance of breeding process (OR=3.061, P=0.003), and decreased with the increase of predator risk (OR=0.451, P=0.023). The intensity of parent bird alarm calls increased with the increase of predator risk and the advance of breeding process (P < 0.001). The nestlings did not respond to the alarm call of the parent birds in the early brooding stage. However, when the alarm call of the parent bird to the chipmunk was heard in the late brooding period, all activities were quickly stopped and squatted silently at the bottom of the nest box (n=31, P < 0.001). When the alarm sound of the parent bird to the Elaphe anomala was heard, 87.1% of the nestlings in the breeding nest would quickly try to escape from the nest box, while the rest of the nestlings were still squatting silently at the bottom of the nest (n=31, P < 0.001).