Ground-level ozone (O₃) has seriously threatened crop production in China, and the fertilization can regulate soil nutrient balance, thereby promoting crop growth. This study was conducted in open top chambers, using two O₃ treatments (ambient air (NF) as control; ambient air+40 nmol/mol O₃ (NF40)). Three fertilizer treatments were nested under each O₃ treatment, including conventional application of inorganic fertilizer (Ino, 270 kg N hm^-2 a^-1), 30% reduction of inorganic fertilizer (Red, 189 kg N hm^-2 a^-1), and combination of organic and inorganic fertilizer (Com, Red+5000 kg hm^-2 a^-1 chicken manure). The photosynthetic parameters of two rice cultivars (HLY898 and NJ9108) at different growth stages were measured, which mainly explored the effects of O₃ on the photosynthetic and physiological processes of rice at different growth stages under different fertilizer treatments. The results showed that NF40 had no significant effect on the saturated photosynthetic rate (A_sat) during the vegetative growth stage of rice, but it significantly reduced the A_sat during the filling stage of rice. Based on the slopes of the relationship between the relative reduction of A_sat and SPAD of two rice cultivars with the O₃ cumulative index, it was found that the hybrid rice cultivar HLY898 (slopes of A_sat and SPAD: -1.55 and -0.98,respectively.) was more sensitive to O₃ than the conventional rice cultivar NJ9108 (slopes of A_sat and SPAD: -0.92 and 0.06,respectively.). In addition, based on the results for the stomatal conductance (g_s) and intercellular carbon dioxide concentration (C_i) of rice under different O₃ treatments, we found that the decrease photosynthesis caused by O₃ in NJ9108 was mainly attributed to non-stomatal limitations, while the decrease in photosynthesis of HLY898 was limited by both stomatal and non-stomatal factors. Compared with the Ino treatment, the Red treatment significantly inhibited A_sat in the two rice cultivars by reducing leaf SPAD, but there was no significant difference in A_sat between the Ino and Com treatments. This suggested that the combined application of organic and inorganic fertilizers could partially alleviate the decrease in rice photosynthesis caused by reduced application of inorganic fertilizers. There was no significant interactive effect of O₃ and fertilizer treatment on all photosynthetic parameters of the two rice cultivars, indicating that short-term combination of organic and inorganic fertilizers could not effectively alleviate the negative effects of O₃ on crops. The research results can provide a theoretical basis for mitigating crop yield reduction caused by O₃ through reasonable nitrogen fertilizer management measures in farmland under O₃ pollution.