The Hubei subspecies of the golden snub-nosed monkey(Rhinopithecus roxellana hubeiensis) is the subspecies with the fewest population among all subspecies of golden snub-nosed monkey(R. roxellana). Estimating their food composition and its seasonal variation contribute to learning their foraging strategy and the competition between the sympatric species which is of great significance to species protection and their habitat restoration. The fresh fecal samples of golden snub-nosed monkeys were collected from Shennongjia National Park in winter (Feb.), spring (May.), and autumn (Oct.) of 2023. The plants for food and seasonal variation in them were analyzed by using DNA metabarcoding technology. A total of 127 plant species were identified, all plants belongs to 110 genera and 62 families. Rosaceae had the highest relative read abundance at the family level, and Sorbus had the highest relative read abundance at the genus level. The highest relative read abundance at the specie level was found in Archiphysalis sinensis. In the food composition of different seasons, 56 species of plants were found in winter, 76 species in spring, and 70 species in autumn. Sorbus sp. (27.34%) and Clematoclethra scandens (16.56%) were the preferred foods in spring. The preferred foods in autumn were Potentilla chinensis (22.12%) and Sorbus sp. (16.52%). In winter, the preferred food were Archiphysalis sinensis (33.49%) and Sorbus sp. (28.13%). There were 20 mutual species in the 3 seasons, 16 endemic species in winter, 29 endemic species in spring, and 27 endemic species in autumn. One of the winter endemic species is Archiphysalis sinensis, one of the spring endemic species is Pimpinella rhomboidea, and one of the autumn endemic species is Broussonetia papyrifera. The maximum number of mutual species was 35 between spring and autumn, 32 mutual species between spring and winter, while the minimum number of mutual species was 28 between winter and autumn. Food diversity index varied between seasons, with the lowest food diversity index in winter, higher in autumn and highest in spring. Seasonal variations in food diversity of golden snub-nosed monkeys are consistent with the phenological characteristics of the vegetation in Shennongjia National Park. The result of study revealed the dietary composition and seasonal variation of golden snub-nosed monkeys in Shennongjia National Park, as well as the advantages and problems of DNA barcoding technology in dietary research, which provided vital reference value for the population breeding, conservation management of the golden snub-nosed monkeys and the others.