Land quality is one of the important factors to ensure food security, and it plays a significant role in maintaining the high-quality development of social economy and the sustainable development of ecosystem. With the rapid economic development and frequent resource exploitation in Yulin, the land quality is seriously degraded. Land degradation is a vital factor which limits the ecological development of Yulin, so it is very important to master the land change from 2000 to 2020. We can use Land degradation neutral (LDN) assessment to reveal land change. In a recent research, a comprehensive LDN assessment rule based on ecosystem service value (ESV) was proposed, so we chose this method to analyze the LDN changes in Yulin City from 2000 to 2020. The research showed that: (1) From 2000 to 2020, as a whole , the general trend of ESV in Yulin City experienced three phases. At first, it rose, then it fell, at last it rose again, with the highest ESV of 23.921 billion in 2020 and the lowest ESV of 23.203 billion in 2000. (2)The LDN assessment based on revised ESV: ①The area of Land-Use and Land-Cover Change (LUCC) stable region is 37261.53km2, which accounted for 86.89%. While , the area of Land-Use and Land-Cover Change (LUCC) recovery region is 2851.75km2, slightly larger than that of the degraded region whose area was 2769.65km2. ②During the twenty years from 2000 to 2020, the net primary productivity (NPP) increased significantly, and areas of increased region, stable region and decreased region were 37501.55 km2, 5870.24 km2, and 93.09 km2, respectively, with very little degradation area. ③ The stable region had the highest area of soil organic carbon (SOC) content, and its area is 37761.65 km2. Compared with that of stable region, the area of recovery region was smaller, it is 2756.83 km2. While, the degraded region area of soil SOC was least, just 2364.47 km2 . ④ We used a combinative way based on LUCC, NPP and SOC to perform a comprehensive analysis of LDN. The result showed that the area of stable region, recovery region and degraded region of LDN in Yulin City was 4764 km2, 35057 km2 and 3046 km2, respectively, with the largest recovery area and the smallest degraded area. The LDN target was achieved in Yulin City from 2000 to 2020. The research results of this paper can provide a theoretical basis for the land use and protection work in Yulin city.