As an important geographical unit for the development of the national economy and society, how to properly address the issue of "tragedy of the commons" within the watershed has always been a major challenge faced by policy makers and academic researchers. How to clarify the rights and responsibilities of ecological compensation in river basins from the perspective of ecological equity and determine the ecological compensation standards and spaces at the township level, is of great significance for breaking the dilemma of sharing compensation funds in river basins and promoting comprehensive ecological compensation. This paper constructs a framework for calculating ecological compensation standards in the Irtysh River Basin based on the value of ecological services. The Least Squares Dummy Variables Regression (LSDV) model is used to determine the compensation standards for the basin, and the compensation priority discrimination method is used to analyze the problem of spatial selection for basin compensation. The results show that: ① From 2000 to 2020, the land use conversion in the Irtysh River Basin showed a characteristic of forest to grass, grass to other, especially from 2000 to 2010, the net transfer scale of forest land reached 2088.34 km², accounting for 7.16% of the total area. The net transfer scale of grassland reached 5916.94 km², accounting for 12.21% of the total area. ② There is a significant two-stage trend between the total compensation amount and the total payment amount in the Irtysh River Basin from 2000 to 2020. The corresponding amount tende to stabilize after 2010, and the compensation intensity in 2020 decreased by 56.06% compared to 2000, while the payment intensity decreased by 23.72%. ③ The ecological compensation spatial zoning in the Irtysh River Basin had a significant north compensation-south payment feature. In 2020, the scale of priority compensation funds in the Irtysh River Basin was 1.453 billion yuan, and the scale of priority payment funds was 1.923 billion yuan. The areas of priority compensation areas and priority payment areas reached 1665 km² and 720 km², respectively. The watershed ecological compensation plan provided in the paper has scientific reference value for diversified ecological compensation work in township units. Relevant departments can improve the accuracy of ecological transfer payments and develop a comprehensive ecological compensation plan recognized by multiple parties based on this.