The soil physicochemical properties and the characteristics of plant community in forest were affected by the behaviors of ungulates such as feeding, trampling and excreting. In this study, we selected a typical coniferous mixed with broad-leaved forest in Qingliangfeng National Nature Reserve of Zhejiang as our research area.We set up four treatments related to Cervus nippon kopschi disturbance (no captivity; 10 a captivity, 15 a captivity and 20 a captivity) to explore their effects on soil properties and plant growth. The results indicated that (1) the values of soil pH, soil water content(SWC), soil capillary porosity (SCP), soil organic matter (SOM) and cation exchange capacity (CEC) tended to decrease with the rise of the captivity years of Cervus nippon kopschi. On the other hand, the contents of bulk density(BD), available nitrogen (AN), available phosphorus (AP) and available potassium (AK) tended to increase with the rise of the captivity years of Cervus nippon kopschi. (2) The soil physicochemical properties parameters (AN and AP of 0-20 cm soil layer, 0-40 cm soil layer: BD, SWC, CP, SOM, CEC) between the treatment with 20 a captivity and the control plots showed significant differences (P < 0.05). (3) Captivity of Cervus nippon kopschi also significantly reduced the aboveground biomass of plants (P < 0.05). This study reveals the response characteristics of soil physiochemical properties and plant aboveground biomass to captive Cervus nippon kopschi in a typical subtropical forest. This study could provide scientific bases of data and theory for exploring the degradation mechanism of ungulates habitat in forest and improving the regional ecological restoration.