Quantification of landscape pattern is the prerequisite for determining the impact of landscape pattern on ecological processes, and is also critical reference for ecological system planning and management. Existing softwares for conducting landscape pattern only provide calculation of landscape pattern indices based on the "Patch-Corridor-Matrix" paradigm. None of them provide calculation for indices based on the "Source-Pathway-Sink" paradigm, which are not catching up with the theoretical development in landscape ecology. In addition, their existing way of implementation and calculation capability cannot meet the requirement of speed and flexibility by the increased size of input data and application situation in the current big data era. Thus, this study developed the landscape ecology toolbox (LET), a new software capable of calculating indices for both paradigms and providing both graphical and command line user interfaces. The paper firstly described the main features of the LET software. Then, the detailed comparison was made on LET with existing software for calculating landscape pattern analysis in terms of their usage, indices provided by each software, and special functions. At last, future development of the LET was discussed.