Soil ecosystems are recognized as crucial energy reservoirs within the biosphere. Stable and healthy soil ecosystems play a pivotal role in maintaining the productivity of terrestrial ecosystems. However, evaluating soil ecosystem stability remains a challenge due to the complexity of biological reactions and soil processes and the difficulty of directly observing subsurface systems' dynamics. For the past few years, the utilization of the soil nematode community maturity index has gained widespread implementation and has been utilised as indicator taxa for the assessment of soil ecosystem stability. Therefore, this study aimed to evaluate soil ecosystem stability within the Lasha Mountain region by employing the soil nematode community maturity index across a total of 52 sample plots from 13 fixed major quadrats, and to verify the feasibility of this evaluation method. Furthermore, we aimed at assessing the practicability of this evaluation system when metagenimics technology was employed. We identified a total of 963 nematode amplicon sequence variants, distributed across 2 classes, 10 orders, 41 families, and 67 genera. Among them, the bacterivorous and omnivorous-predatory nematodes emerged as the dominant groups, with the majority of them displaying K-strategy characteristics (c-p 3-5). The average soil nematode community maturity index was measured at 3.24±0.32, suggesting that the soil ecosystem was relatively stable, while the spatial distribution of the soil nematode community maturity index in Lasha Mountain area exhibited no significant variation (P>0.05). A significantly negative correlation was found between the artificial disturbance intensity obtained by using the plot monitoring and the results evaluated by using the soil nematode community maturity index (P<0.05), indicating that the plots with low stability also showed greater interference with human activities. Concurrently, the assessment results of the soil nematode maturity index and the validations of surface ecosystems of the sample plot revealed an elevational pattern, with mid-altitude regions showing lower nematode community maturity index values, attributed mainly to the deep river valleys and extreme steepness of the slopes on both sides characteristic of the region which experiences frequent landslides. This particular situation results in soil ecosystem instability and the loss of soil nematode community maturity index. These assessment outcomes align with the real conditions of the surface habitat. Consequently, it reaffirms the feasibility of the soil nematode community maturity index as an assessment tool for soil ecosystems stability, potentially paving the way for its application in the evaluation of regional ecosystem health in future assessments.