Abstract:In order to confirm the ecological restoration function of slope microtopographic modification measure-contour reverse-slope terrace (CRT) on sloping farmland, it is extremely critical to reveal the effects and mechanisms of CRT on the glomalin-related soil protein (GRSP) content and contribution to soil carbon and nitrogen storage. The total GRSP (T-GRSP), easily extractable GRSP (EE-GRSP), soil organic carbon (SOC), and total nitrogen (TN) contents were determined in the soil of two terrace positions (above and below the terrace) and three depths (0-10, 5-15 and 15-30 cm) of CRT and the corresponding positions of unmodified (CK) sloping farmland. The contribution of GRSP to soil carbon and nitrogen storage under CRT was explored, and the relevant mechanism was revealed. The results showed that: (1) comparing with CK, the contents of T-GRSP, EE-GRSP, SOC, and TN in CRT significantly increased by 16.6%-189%, 7.28%-102%, 1%-68.3%, and 7.29%-79.7%, respectively (P<0.05). In addition, the promotion of CRT on GRSP content in sloping farmland was generally better in the rainy season than that in the dry season, higher in the surface soil (0-5 cm) than that in the deep soil (5-15 cm), and better in the slope below the terrace than that above the terrace. (2) Comparing with CK, SOC and TN storage in CRT sloping farmland increased by 8.06% vs. 13.5% and 7.01% vs. 12.1% in the dry and rainy seasons, respectively. Comparing with CK, the contribution rate of T-GRSP to SOC and TN in CRT increased by 8.5%-141% and 2.58%-133%, meanwhile, the contribution rate of EE-GRSP to SOC and TN in CRT increased by 1.38%-82% and 5.25%-87.2%. Moreover, the enhancement of CRT on SOC and TN storage in the rainy season was better than that in the dry season, and the enhancement of CRT on the contribution rate of T-GRSP to SOC and TN in surface soil was stronger in the rainy season than that in the dry season, and stronger in the slope below the terrace than that above the terrace. (3) CRT measures greatly improved the positive correlations between T-GRSP and soil porosity, SOC, and TN comparing with CK, while the correlation coefficients (R2) increased from 0.17, 0.26, and 0.29 to 0.51, 0.66, and 0.64, respectively. The results indicated that CRT measures not only reduced the loss of SOC and TN in sloping farmland, but also improved the soil aeration and permeability by increasing the content of organic matter, thus increasing the secretion and accumulation of GRSP in sloping farmland. In addition, soil carbon and nitrogen retention and storage were promoted through increasing the contribution rate of GRSP to carbon and nitrogen by CRT measures.