Research on the influence of change of organic carbon input from aboveground litter and belowground roots on soil biological community is a topical issue in academic circles at present. However, it is unclear about the influence of organic carbon input methods on soil fungal community structure and functional group during the freeze-thaw season. The soil fungal community is an important factor in regulating the stability of forest ecosystem, which help to maintain the stability of ecosystem productivity timescales. In order to investigate the response characteristics of the soil fungal community to organic carbon input from aboveground litter and belowground root in freeze-thaw seasonal temperate forest, this study used ITS rDNA high-throughput sequencing technology and the FUNGuild functional prediction platform. Four different carbon input treatments were set up in Maoershan Ecological Station(Detritus input and removal treatments,DIRT), including litter removal (NL), root removal (NR), litter and root removal (NLR) and control(CK). We analyzed the influence of controlling roots and litter on fungal community structure and functional groups in temperate forest soil. The result showed that: (1) different organic carbon input patterns altered the relative abundance of the soil fungal community. Root organic carbon input had more obvious effects on the relative abundance of soil fungal communities than litter inputs. Relative abundance of the Ascomycota was 19.52% higher and relative abundance of the Basidiomycota was 16.77% lower than those in the control treatment, respectively.(2) The mode of organic carbon input had a significant effect on the functional group of the soil fungal community. The proportion of Saprotroph fungi and Pathotroph fungi were 9.79% and 1.22% higher in root removal treatment, at the same time which were 7.58% and 0.85% lower than those in the control treatment. (3) The influence of organic carbon input mode on fungal community structure was related to soil physical and chemical environmental factors. The content of microbial nitrogen was a key factor leading to the emergence of significant differences in soil fungal community structure. The results highlighted the importance and regulation of organic carbon inputs on soil fungal community structure and functional group. The results enriched the research content of soil microbial community in temperate forest, and provided theoretical support and reference for the study of the influences of soil microecological environment changes on ecosystem processes. In addition, the future studies should focus on the long-term effects of litter and root input treatments on soil fungal community in different seasons.