As a new green infrastructure and nature-based solution, green roofs have become an important ecological strategy to enhance urban ecological resilience and maintain biodiversity. However, urban ecological network studies mostly focus on 2D ground ecological space, and few studies focus on the construction of 3D ecological network considering green roofs. The studies of 3D ecological network that integrate ground and roof space and take green roofs into account are much less common. Green roofs can play an important role in improving landscape connectivity between fragmented urban ecological patches and optimizing the 3D urban ecological network. Therefore, it is necessary to integrate ground and rooftop ecological spaces, and to conduct research on the construction of urban 3D ecological networks that take green roofs into account, to fulfill the effective connection between urban green roof ecosystems and ground ecological spaces. In this paper, based on the data of LiDAR, high-resolution remote sensing, and building attributes, we firstly structured a framework to integrate urban 3D ecological networks and green roofs by the multi-factor superposition and circuit theory. We used multi-factor superposition to analyze the suitability of green roofs in four aspects:building height, roof slope, building age, and historical and cultural attributes. Secondly, combining 3D structural features data of buildings and vegetation, we constructed the ecological networks under two scenarios with or without green roofs by the method of circuit theory. Finally, the potential impact of green roofs on urban 3D ecological networks was analyzed by comparing the number, length and average resistance of ecological corridors. The results showed that:1) There were 2879.62 hm² of roofs in the study area suitable for greening, accounting for 46.59% of the total roof area in the study area. It indicated that green roofs had great potential for development. 2) The number of ecological corridors considering green roofs increased from 205 to 209, the total length of the ecological network increased and the resistance value per unit length decreased, indicating that green roofs could significantly improve the landscape connectivity of urban ecological spaces, increase the density of ecological corridors, and provide important habitats and stepping stones for high-mobility species. 3) The effects of green roofs on ecological networks were significantly regional different, with the largest optimization effect in the central urban area, followed by the industrial area, and few optimization effects in ecologically sound areas. The results of the study can provide decision basis and reference information for analysis and planning of ground ecological space and green roof space in high-density urban areas.