Biodiversity maintenance is an important ecological function in the Three\\River Headwater Region (TRHR). After the implementation of a series of ecological projects such as the construction of nature reserves, the population of large wild herbivores in TRHR has recovered. In order to further promote biodiversity conservation and formulate comprehensive scientific plans for various conservation objectives, it is necessary to know the habitat distribution and resource competition relationships of wild animals in the area. Compared with traditional ground survey methods, UAV remote sensing has the advantages of flexibility, simplicity, low cost, adaptability to complex terrain, etc. And the acquired data has geospatial attributes, so that it can be integrated with 3S technology for spatial superposition analysis, which has application potential in the field of wildlife habitat investigation. In this study, UAV remote sensing technology was used to identify the suitable habitats of Kiang (Asinus kiang), Tibetan gazelle (Procapra picticaudata), and Blue sheep (Pseudois nayaur) by using selection index, as well as the competition relationship of habitat resources utilization by using niche overlap degree in Maduo County. The results showed that: (1) a total of 252 Kiangs were found in the UAV transects, with an average population density of 0.77 /km². And 199 Tibetan gazelles were found, the average population density was 0.61 /km². There were also 203 Blue sheep, with an average population density of 0.62 /km² in the UAV transects. (2) The Kiangs preferred to choose the area with slope less than 5°, above 1 km from rural settlements and roads and within 1 km from water as habitat. The Tibetan gazelle preferred habitat areas with slope between 2 -10°, vegetation coverage greater than 0.6, within 1-3 km from the water source, and with vegetation type of kobresia pygmaea (Carex parvula O. Yano)+forb, kobresia pygmaea and stipa purpurea (Stipa purpurea Griseb.). The Blue sheep preferred to choose the habitat area of stipa purpurea+forb and kobresia pygmaea meadow with slope greater than 5°, more than 3 km away the road and within 1-3 km from the water. (3) The suitable habitat area of Kiang in Madao County was 3559.43 km², and the distance from water source was the main factor limiting the suitable habitat area. The suitable habitat area of Tibetan gazelle was 283.76 km², and the suitable habitat area of Blue sheep was 29.97 km², the suitable habitat area of which was both limited by the slope, distance from water source and vegetation type. Due to niche differences in habitat selection of slope, the habitat overlap area of the three large wild herbivores was small, and the habitat competition was weak. This study provides a new idea for biodiversity conservation and can support the comprehensive planning of wildlife conservation and restoration on the Qinghai-Tibetan Plateau and similar areas.