Population behavior differentiation is the basis for species to adapt to the environment, while the differentiation of geographic-based activity rhythms of various populations that belong to one species is an essential part of ecological adaptation. Such geographic-varied activity rhythms of species populations are mainly determined by the ecological conditions and interspecific competition. Thus, understanding the geographic-differentiated activity rhythm can contribute to the management and protection of populations in a dynamic manner. In order to study the annually behavioral patterns of different populations that dwell geographical differently, population data of Silver pheasant (Lophura nycthemera) was collected from sixty infrared cameras (from October 2017 to September 2018) were utilized in Hunan Dupangling National Nature Reserve and Hunan Liuyang Daweishan Provincial Nature Reserve, respectively. The kernel density estimation method and the Jacobs Selection Index (JSI) were adopted to construct and compare the daily activity rhythm and the daily time nodes for this species, while the differentiation trends of activity rhythm between the two populations were analyzed with Pearson correlation coefficient and T-test. The findings indicated a single-peak mode of the daily rhythm of silver pheasant in Dupangling all through the year and in cold season, and a dual-peak mode in the warm season. Notably, an obvious seasonal reversal was characterized with continuous multi-peak throughout the year, and a single-peak mode in both cold and warm seasons for Silver pheasant in Daweishan. In terms of time selection, Silver pheasants in Dupangling were more likely active in the morning through the whole year and the warm year (JSI=0.497, JSI=0.461), and tended to be more active at midday in the warm season (JSI=0.593). In addition, sunsets were negative choices throughout the year and different seasons (JSI<0) in this population. However, Silver pheasants in the Daweishan were more frequently active in afternoon all around the year and the cold year (JSI=0.454, JSI=0.673), and preferred to be active in the morning (JSI=0.423) in the warm season. While, sunsets were positive choices by these populations in the warm season (JSI=0.082). According to the activity time overlap coefficient, the results showed the overlap coefficient reached the highest degree (Dhat=0.92) between the two geographic populations of Silver pheasant in the warm season, in which the activity frequency of the two geographical populations was the most differentiated by activity frequency correlation analysis (Cor=-0.92, P<0.01). Comprehensive analysis suggested that the reasons for the activity rhythm differentiation of Silver pheasants were temperature adaptation, human interference avoidance, and abundance pressure of competitors and predators in Dupangling and Daweishan. The study provided both empirical clue for the future research that explores the daily activity rhythm differentiation of pheasants and theoretical support for the population management and habitat protection of silver pheasant.