降水变化对高寒草甸土壤线虫群落的影响
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国家自然科学基金项目(U20A2008,41971064);第二次青藏高原综合科学考察研究项目(2019QZKK0302);西南民族大学研究生创新项目(2021NYYXS12)


Effects of altered precipitation on soil nematode communities in an alpine meadow
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(U20A2008、41971064)、(2019QZKK0302)、(2021NYYXS12)

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    摘要:

    土壤线虫是土壤食物网结构和功能的重要指示生物,研究降水变化对高寒草甸土壤线虫群落的影响可以了解高寒草甸地下食物网结构和功能对气候变化的响应。2015年12月在川西北高寒草甸设置了3个减水处理(-90%、-50%、-30%)、1个自然降水(CK)和1个增水处理(+50%)共5个降水梯度的实验,各梯度对应的年降水量依次为88 mm、442 mm、618 mm、883 mm和1325 mm左右。2020年9月对各处理样地的土壤线虫群落、植物和土壤环境因子进行了调查,用Baermann法分离土壤线虫,并对土壤线虫的群落组成结构、密度、多样性和生态指数等进行了分析。结果表明:(1)随年降水量的增加,土壤线虫群落组成结构和密度发生显著变化,多样性指数无显著变化;食真菌线虫相对密度显著降低,而植物寄生线虫相对密度显著增加;(2)当年降水量小于442 mm时,线虫群落密度及各营养类群线虫密度均显著下降;年降水量大于442 mm时,食真菌线虫密度显著下降,植物寄生线虫密度显著增加,以食细菌和食真菌线虫为主的营养结构转变为以食细菌和植物寄生线虫为主;(3)当年降水量大于883 mm时,土壤线虫群落密度无显著变化,但0-10 cm土层的通道指数小于50,土壤有机质的细菌分解途径功能得到增强,不利于土壤碳及养分累积;(4)年降水量变化及其引起的土壤含水量、全氮和pH变化是影响土壤线虫群落的主要因子。研究结果表明,如果未来气候变化导致青藏高原东缘变得更湿润,对高寒草甸生态系统的线虫群落密度及多样性影响较弱,但对土壤食物网结构及功能影响较强。

    Abstract:

    The Qinghai-Tibetan Plateau, the third polar of Earth, responds sensitively to global climate change. Soil nematodes are important indicators of the structure and function of soil food web. However, few studies have been conducted on the effects of altered precipitation on the taxonomic composition and diversity of soil nematode community in alpine meadows on the Qinghai-Tibetan Plateau. Therefore, understanding of the impacts of climate change on the structure and function of the soil food web in alpine meadows was inadequate at present. To explore the effects of altered precipitation on soil nematode communities in alpine meadows, the precipitation experiment, including three reduction precipitation treatments (-90%, -50%, and -30%), a control treatment (CK, natural precipitation), and an addition precipitation treatment (+50%), was set up in an alpine meadow in northwest Sichuan in December 2015. The annual precipitations of the five treatments were 88 mm, 442 mm, 618 mm, 883 mm, and 1325 mm, respectively. The soil nematode communities and the environmental factors of plant and soil were investigated from the plots of the five precipitation treatments in September 2020. Soil nematodes were extracted from 50 g of fresh soil using Baermann funnels, and the changes in the taxonomic composition structure, abundance, diversity index, and ecological index were analyzed. The results showed that:(1) the taxonomic composition and abundance of the soil nematode communities changed significantly with the increased annual precipitation and no significant changes in the diversity index. The relative abundances decreased significantly for fungivores and increased significantly for plant parasites with the increased annual precipitation. (2) The abundances of soil nematode community and four trophic groups decreased significantly when the annual precipitation was less than 442 mm. The abundances decreased significantly for fungivores and increased significantly for plant parasites when the annual precipitation was more than 442 mm, resulting into the dominating trophic groups shifted from the bacterivores and fungivores to the bacterivores and plant parasites. (3) When the annual precipitation was more than 883 mm, the abundance of soil nematode community varied with no significance, but the channel index of the top 10 cm soil layer was smaller than 50, indicating that the function of bacterial decomposition pathway for the soil organic matter was strengthened, which was unfavorable to the accumulation of soil carbon and nutrients. (4) The changes in the contents of soil moisture and total nitrogen and pH induced by the altered annual precipitation were the determining drivers for the soil nematode communities. The results suggest that the abundance and diversity of soil nematode community in alpine meadows will be affected weakly, but the structure and function of soil food web will be affected strongly when the climate becomes more humid on the eastern edge of Qinghai-Tibetan Plateau in the future.

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代江慧,吴鹏飞,唐思思,王长庭,王玉英,任晓,魏雪.降水变化对高寒草甸土壤线虫群落的影响.生态学报,2023,43(22):9371~9383

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