1.生态环境部南京环境科学研究所国家环境保护生物多样性与生物安全重点实验室;2.生态环境部南京环境科学研究所 国家环境保护生物多样性与生物安全重点实验室;3.复旦大学 生物多样性科学研究所
1.State Environmental Protection Key Laboratory on Biodiversity and Biosafety,Nanjing Institute of Environmental Science,Ministry of Ecology and Environment;2.Institute of Biodiversity Science,Fudan University
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
两栖动物是我国受威胁程度最高的动物类群，加强两栖动物资源调查和多样性监测，是开展两栖动物保护和濒危物种拯救行动的关键性基础工作。传统的两栖动物监测主要以形态学和声学为基础，耗时费力，且难以发现一些隐蔽性较强的稀有物种。基于环境DNA(environmental DNA, eDNA)的调查方法以其快速、灵敏、高效、无创等独特优势，为两栖动物多样性监测及保护提供了新的工具。本文综述了eDNA在两栖动物多样性监测、外来入侵和珍稀濒危物种调查、物种丰度或生物量估测等研究领域的应用进展，分析了两栖动物eDNA产生、扩散、迁移和降解的动态变化特征及其关键影响因子，探讨了eDNA应用于两栖动物监测研究的局限性并提出了优化建议，同时对未来的研究方向进行了展望，以充分挖掘eDNA在两栖动物监测中的应用潜力，为两栖动物多样性保护和管理提供新的思路。
Amphibians have a unique life history and are?extremely sensitive to habitat changes. They are important indicator groups for monitoring environmental quality changes and assessing ecosystem health. Amphibians have become the most threatened animal group in China due to habitat?loss?and?degradation, environmental pollution, climate change, overharvesting,?invasion?of?alien?species, and biological internal?factors. Strengthening of resource surveys and population monitoring of amphibians constitute the fundamental work for amphibian diversity protection and endangered species rescue.?Traditional amphibian monitoring is mainly based on morphology and acoustics, which is time-consuming, laborious, and highly dependent on professional classification knowledge and investigation experience. Furthermore, it is difficult to find some rare species given their strong concealment. The investigation method based on environmental DNA (eDNA) has unique advantages. It is fast, sensitive, efficient, non-invasive, low cost, and independent of traditional classification experience, etc. This can make up for the shortcomings of traditional investigation methods and provide a new tool for amphibian diversity monitoring and protection. eDNA has been successfully applied in amphibian research, such as monitoring alien invasive species, rare and endangered species protection, biodiversity assessment, and species abundance or biomass estimation. However, in practical application research, due to different application environments, target?organisms, sampling protocols, and primer selection, the results?of?the experiment?were extremely varied. Although previous studies have promoted the progress of this technology, they could not provide systematic guidance for amphibian investigation and monitoring, and there is still uncertainty in the complex ecological processes of eDNA from amphibians. In this review, we summarized the application of eDNA in amphibian monitoring. We analyzed the critical factors that should be considered in its application, including the shedding and capture of eDNA, the selection of sampling location and time, the transport and deposition of eDNA, and the degradation of eDNA, as well as the?associated biotic and abiotic factors. In addition, we discussed the limitations of the application of eDNA and provided several optimization?suggestions for improving the efficiency and accuracy of the amphibian monitoring base on eDNA. Finally, we looked toward future research directions to fully exploit?the application potential of eDNA in amphibian monitoring and provide new ideas for amphibian diversity protection and management.
肖泽华,董姗姗,张振华,章嫡妮,宋志平.环境DNA在两栖动物监测中的应用研究进展.生态学报,,(). http://dx. doi. org/[doi]复制